PPP2R5D antibody
Goat anti PPP2R5D
PPP2R5D antibody recognizes serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform, also known as PP2A B subunit isoform B'-delta, or PP2A B subunit isoform R5-delta. The antibody has been extensively validated for western blotting using whole cell lysates.
PPP2R5D Antibody with Control Lysate
PPP2R5D antibody recognizes serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform, also known as PP2A B subunit isoform B'-delta, PP2A B subunit isoform B56-delta, PP2A B subunit isoform PR61-delta or PP2A B subunit isoform R5-delta.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00028 | WB | 100 µl | ||||
| VPA00028KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human PPP2R5D antibody recognizes human serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform, also known as PPP2R5D, PP2A B subunit isoform B56-delta or PP2A B subunit isoform PR61-delta. PPP2R5D is a 602 amino acid phosphatase. Three isoforms of PPP2R5D are generated by alternative splicing (Tanabe et al. 1997), all isoforms retain the C terminal region. Isoform-1 is widely expressed while isoform-2 is restricted to the brain.
Goat anti Human PPP2R5D antibody recognizes human PPP2R5D as a band of ~75 kDa in multiple cell line lysates by western blotting under reducing conditions. The antibody also cross reacts with PPP2R5D from rodent cell line lysates tested.
Goat anti Human PPP2R5D antibody recognizes human PPP2R5D as a band of ~75 kDa in multiple cell line lysates by western blotting under reducing conditions. The antibody also cross reacts with PPP2R5D from rodent cell line lysates tested.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
- Preparation
- 20μl Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Peptide sequence C-KRAEEFLTASQEAL from the C-terminal region of PPP2R5D
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q14738 Related reagents
- Entrez Gene
- PPP2R5D Related reagents
- GO Terms
- GO:0000159 protein phosphatase type 2A complex
- GO:0005515 protein binding
- GO:0007165 signal transduction
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0007399 nervous system development
- GO:0008601 protein phosphatase type 2A regulator activity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of PPP2R5D antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
- Western Blotting
- Anti PPP2R5D antibody detects a band of approximately 75 kDa in HEK293 cell lysates.
- Western Blotting
- Anti PPP2R5D antibody detects a band of approximately 75 kDa in HEK293 cell lysates.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
