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PPP2R5D antibody

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Western Blot Validated
Anti PPP2R5D Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with PPP2R5D antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 60 second exposure. Arrow points to PPP2R5D (molecular weight 75 kDa)

Goat anti PPP2R5D

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00028
Datasheet
WB
MSDS
100 µl

Goat anti Human PPP2R5D antibody recognizes human serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform, also known as PPP2R5D, PP2A B subunit isoform B56-delta or PP2A B subunit isoform PR61-delta. PPP2R5D is a 602 amino acid phosphatase. Three isoforms of PPP2R5D are generated by alternative splicing (Tanabe et al. 1997), all isoforms retain the C terminal region. Isoform-1 is widely expressed while isoform-2 is restricted to the brain.

Goat anti Human PPP2R5D antibody recognizes human PPP2R5D as a band of ~75 kDa in multiple cell line lysates by western blotting under reducing conditions. The antibody also cross reacts with PPP2R5D from rodent cell line lysates tested.

Product Details

Target Species
Human
Western Blotting
Anti PPP2R5D antibody detects a band of approximately 75 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-KRAEEFLTASQEAL from the C-terminal region of PPP2R5D

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
Q14738
Entrez Gene
PPP2R5D
GO Terms
GO:0000159 protein phosphatase type 2A complex
GO:0005515 protein binding
GO:0007165 signal transduction
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0007399 nervous system development
GO:0008601 protein phosphatase type 2A regulator activity
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PPP2R5D antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
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