PPP2R5D antibody

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Goat anti PPP2R5D

PPP2R5D antibody recognizes serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform, also known as PP2A B subunit isoform B'-delta, or PP2A B subunit isoform R5-delta. The antibody has been extensively validated for western blotting using whole cell lysates.

PPP2R5D Antibody with Control Lysate

PPP2R5D antibody recognizes serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform, also known as PP2A B subunit isoform B'-delta, PP2A B subunit isoform B56-delta, PP2A B subunit isoform PR61-delta or PP2A B subunit isoform R5-delta.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00028 WB 100 µl
VPA00028KT WB 2 Westerns
With Lysate 400µg
Goat anti Human PPP2R5D antibody recognizes human serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform, also known as PPP2R5D, PP2A B subunit isoform B56-delta or PP2A B subunit isoform PR61-delta. PPP2R5D is a 602 amino acid phosphatase. Three isoforms of PPP2R5D are generated by alternative splicing (Tanabe et al. 1997), all isoforms retain the C terminal region. Isoform-1 is widely expressed while isoform-2 is restricted to the brain.

Goat anti Human PPP2R5D antibody recognizes human PPP2R5D as a band of ~75 kDa in multiple cell line lysates by western blotting under reducing conditions. The antibody also cross reacts with PPP2R5D from rodent cell line lysates tested.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-KRAEEFLTASQEAL from the C-terminal region of PPP2R5D

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q14738 Related reagents
Entrez Gene
PPP2R5D Related reagents
GO Terms
GO:0000159 protein phosphatase type 2A complex
GO:0005515 protein binding
GO:0007165 signal transduction
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0007399 nervous system development
GO:0008601 protein phosphatase type 2A regulator activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PPP2R5D antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti PPP2R5D antibody detects a band of approximately 75 kDa in HEK293 cell lysates.
Western Blotting
Anti PPP2R5D antibody detects a band of approximately 75 kDa in HEK293 cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB