PPP2R5D antibody

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Anti PPP2R5D Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PPP2R5D antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 60 second exposure. Arrow points to PPP2R5D (molecular weight 75 kDa)

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Goat anti PPP2R5D

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: PPP2R5D

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Goat anti Human PPP2R5D antibody recognizes human serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform, also known as PPP2R5D, PP2A B subunit isoform B56-delta or PP2A B subunit isoform PR61-delta. PPP2R5D is a 602 amino acid phosphatase. Three isoforms of PPP2R5D are generated by alternative splicing (Tanabe et al. 1997), all isoforms retain the C terminal region. Isoform-1 is widely expressed while isoform-2 is restricted to the brain.

Goat anti Human PPP2R5D antibody recognizes human PPP2R5D as a band of ~75 kDa in multiple cell line lysates by western blotting under reducing conditions. The antibody also cross reacts with PPP2R5D from rodent cell line lysates tested.

Target Species: Human
Western Blotting: Anti PPP2R5D antibody detects a band of approximately 75 kDa in HEK293 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin
Immunogen: Peptide sequence C-KRAEEFLTASQEAL from the C-terminal region of PPP2R5D
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Donkey anti Sheep/Goat IgG:HRP	STAR88P	C E P WB	1 ml	Log in	Get Quote
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Description	Donkey anti Sheep/Goat IgG:HRP

UniProt: Q14738
Entrez Gene: PPP2R5D
GO Terms: GO:0000159 protein phosphatase type 2A complex; GO:0005515 protein binding; GO:0007165 signal transduction; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0007399 nervous system development; GO:0008601 protein phosphatase type 2A regulator activity