PPP2R1A antibody

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Goat anti PPP2R1A

PPP2R1A antibody recognizes serine/threonine-protein phosphatase 2a 65 kDa regulatory subunit a alpha isoform, also known as protein phosphatase 2, PP2A subunit A isoform PR65-alpha, or PP2A subunit A isoform R1-alpha.

PPP2R1A Antibody with Control Lysate

PPP2R1A antibody recognizes serine/threonine-protein phosphatase 2a 65 kDa regulatory subunit a alpha isoform, also known as protein phosphatase 2, PP2A subunit A isoform PR65-alpha, or PP2A subunit A isoform R1-alpha.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00194 WB 100 µl
VPA00194KT WB 2 Westerns
With Lysate 400µg
Goat anti Human PPP2R1A antibody recognizes PPP2R1A, also known as PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha, medium tumor antigen-associated 61 KDa protein, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform, serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alpha isoform and serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform.

The PPP2R1A gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. PPP2R1A encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described (provided by RefSeq, Apr 2010).

Goat anti Human PPP2R1A antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PPP2R1A detects a band of approximately 60 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
Tris buffered saline
Buffer Solution
Tris buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide with sequence C-KYFAQEALTVLSLA
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P30153 Related reagents
Entrez Gene
PPP2R1A Related reagents
GO Terms
GO:0007059 chromosome segregation
GO:0000086 G2/M transition of mitotic cell cycle
GO:0000159 protein phosphatase type 2A complex
GO:0000188 inactivation of MAPK activity
GO:0000775 chromosome, centromeric region
GO:0016020 membrane
GO:0003823 antigen binding
GO:0005739 mitochondrion
GO:0005829 cytosol
GO:0005625 soluble fraction
GO:0005634 nucleus
GO:0006275 regulation of DNA replication
GO:0006461 protein complex assembly
GO:0006470 protein dephosphorylation
GO:0006672 ceramide metabolic process
GO:0006917 induction of apoptosis
GO:0008380 RNA splicing
GO:0008601 protein phosphatase type 2A regulator activity
GO:0010033 response to organic substance
GO:0015630 microtubule cytoskeleton
GO:0019932 second-messenger-mediated signaling
GO:0030111 regulation of Wnt receptor signaling pathway
GO:0030155 regulation of cell adhesion
GO:0030308 negative regulation of cell growth
GO:0042518 negative regulation of tyrosine phosphorylation of Stat3 protein
GO:0045449 regulation of transcription
GO:0045595 regulation of cell differentiation
GO:0046982 protein heterodimerization activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PPP2R1A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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