PPP1R12A (pSer668) antibody

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Rabbit anti PPP1R12A (pSer668)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2705
Datasheet
WB
MSDS
50 µl loader

Rabbit anti PPP1R12A (pSer668) antibody recognizes human protein phosphatase regulatory subunit 12A (PPP1R12A), also known as MBS or MYPT1, when phosphorylated at serine 668. PPP1R12A is the main regulatory subunit of myosin phosphatases (MPs ) (Butler, T. et al. 2013). PKA and/or PKG phosphorylate PPP1R12A at serine 668, serine 692, serine 695, and serine 852 in smooth muscle. Insulin stimulates the phosphorylation of PPP1R12A at serine 668 (and serine 477, serine 478, serine 507 and serine 695) implying a role for PPP1R12A and these phosphorylation events in insulin signal transduction (Chao, A. et al. 2012).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to PPP1R12A (pSer668) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 668 of human PPP1R12A

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O14974 Related reagents
Entrez Gene
PPP1R12A Related reagents
GO Terms
GO:0005515 protein binding
GO:0004871 signal transducer activity
GO:0005737 cytoplasm
Regulatory
For research purposes only

Applications of PPP1R12A (pSer668) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml