PPP1R12A (pSer668) antibody

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Anti PPP1R12A (pSer668) Antibody gallery image 1 — Western blot analysis of HeLa, Jurkat, NIH/3T3 and C6 cell lysates, untreated or treated with Calyculin A using Rabbit anti PPP1R12A (pSer668) antibody at a 1/1000 dilution. Cells were cultured in serum free media overnight before treatment with Calyculin A (100nM) for 30 minutes. 3% BSA was used for blocking

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Rabbit anti PPP1R12A (pSer668)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: PPP1R12A (pSer668)

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AHP2705 Datasheet Datasheet SDS SDS	WB	50 µl		Log in	Get Quote
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Rabbit anti PPP1R12A (pSer668) antibody recognizes human protein phosphatase regulatory subunit 12A (PPP1R12A), also known as MBS or MYPT1, when phosphorylated at serine 668. PPP1R12A is the main regulatory subunit of myosin phosphatases (MPs ) (Butler, T. et al. 2013). PKA and/or PKG phosphorylate PPP1R12A at serine 668, serine 692, serine 695, and serine 852 in smooth muscle. Insulin stimulates the phosphorylation of PPP1R12A at serine 668 (and serine 477, serine 478, serine 507 and serine 695) implying a role for PPP1R12A and these phosphorylation events in insulin signal transduction (Chao, A. et al. 2012).

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to PPP1R12A (pSer668) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide
50% Glycerol
Immunogen: Phospho specific-peptide corresponding to residues surrounding serine 668 of human PPP1R12A
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Mouse
Rat

Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/500	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description	TidyBlot Western Blot Detection Reagent:HRP