PPP1CC antibody

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Rabbit anti PPP1CC

Rabbit anti human PPP1CC antibody recognizes the serine/threonine-protein phosphatase PP1-gamma catalytic subunit, also known as protein phosphatase 1. This antibody has been extensively validated for western blotting using whole cell lysates.

PPP1CC Antibody with Control Lysate

Rabbit anti human PPP1CC antibody recognizes the serine/threonine-protein phosphatase PP1-gamma catalytic subunit, also known as protein phosphatase 1, catalytic subunit, gamma isoform or protein phosphatase 1C catalytic subunit.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00357 WB 100 µl
VPA00357KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human PPP1CC antibody recognizes PPP1CC, also known as protein phosphatase 1C catalytic subunit, serine/threonine phosphatase 1 gamma and serine/threonine-protein phosphatase PP1-gamma catalytic subunit.

The protein encoded by the PPP1CC gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. PPP1CC encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for PPP1CC (provided by RefSeq, Oct 2011).

Rabbit anti Human PPP1CC antibody detects a band of 37 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PPP1CC detects a band of approximately 37 kDa in HEK293 cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - liquid
Product Form
Ig fraction - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Preparation
20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 291-321 of human PPP1CC
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P36873 Related reagents
Entrez Gene
PPP1CC Related reagents
GO Terms
GO:0005515 protein binding
GO:0000236 mitotic prometaphase
GO:0000777 condensed chromosome kinetochore
GO:0005829 cytosol
GO:0004722 protein serine/threonine phosphatase activity
GO:0046872 metal ion binding
GO:0005730 nucleolus
GO:0005977 glycogen metabolic process
GO:0016607 nuclear speck
GO:0019433 triglyceride catabolic process
GO:0019901 protein kinase binding
GO:0030496 midbody
GO:0032154 cleavage furrow
GO:0051301 cell division
GO:0070688 MLL5-L complex
GO:0072357 PTW/PP1 phosphatase complex
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PPP1CC antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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