PPP1CC antibody
Rabbit anti PPP1CC
Rabbit anti human PPP1CC antibody recognizes the serine/threonine-protein phosphatase PP1-gamma catalytic subunit, also known as protein phosphatase 1. This antibody has been extensively validated for western blotting using whole cell lysates.
PPP1CC Antibody with Control Lysate
Rabbit anti human PPP1CC antibody recognizes the serine/threonine-protein phosphatase PP1-gamma catalytic subunit, also known as protein phosphatase 1, catalytic subunit, gamma isoform or protein phosphatase 1C catalytic subunit.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00357 | WB | 100 µl | ||||
| VPA00357KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human PPP1CC antibody recognizes PPP1CC, also known as protein phosphatase 1C catalytic subunit, serine/threonine phosphatase 1 gamma and serine/threonine-protein phosphatase PP1-gamma catalytic subunit.
The protein encoded by the PPP1CC gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. PPP1CC encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for PPP1CC (provided by RefSeq, Oct 2011).
Rabbit anti Human PPP1CC antibody detects a band of 37 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by the PPP1CC gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. PPP1CC encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for PPP1CC (provided by RefSeq, Oct 2011).
Rabbit anti Human PPP1CC antibody detects a band of 37 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti PPP1CC detects a band of approximately 37 kDa in HEK293 cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Ig fraction - liquid
- Product Form
- Ig fraction - liquid
- Preparation
- Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Preparation
- 20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH-conjugated synthetic peptide corresponding to aa 291-321 of human PPP1CC
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P36873 Related reagents
- Entrez Gene
- PPP1CC Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0000236 mitotic prometaphase
- GO:0000777 condensed chromosome kinetochore
- GO:0005829 cytosol
- GO:0004722 protein serine/threonine phosphatase activity
- GO:0046872 metal ion binding
- GO:0005730 nucleolus
- GO:0005977 glycogen metabolic process
- GO:0016607 nuclear speck
- GO:0019433 triglyceride catabolic process
- GO:0019901 protein kinase binding
- GO:0030496 midbody
- GO:0032154 cleavage furrow
- GO:0051301 cell division
- GO:0070688 MLL5-L complex
- GO:0072357 PTW/PP1 phosphatase complex
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of PPP1CC antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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