Rabbit anti Human PPAR gamma antibody recognises human Peroxisome proliferator-activated receptor gamma (PPARγ) a 57.6 kDa transcription factor belonging to the nuclear hormone receptor (NR1) family.
PPARγ is vital in the regulation of cellular differentiation, development and metabolism. PPARγ is a key regulator of adipocyte differentiation and glucose homeostasis. In addition, PPARγ is the main target of thiazolidinediones (TZDs), a class of drug used in diabetes mellitus and other diseases that feature insulin resistance.
PPARγ is widely expressed with high concentrations being found in adipose tissue.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human PPAR gamma was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Synthetic peptide corresponding to a region near the amino terminus of human PPAR gamma 2.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of PPAR Gamma antibody
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human prostate
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for PPAR Gamma antibody
Kolben, T.M. et al. (2018) PPARgamma Expression is Diminished in Macrophages of Recurrent Miscarriage Placentas
Placentas. Preprints 2018, 2018050351.