PP1 Alpha (pThr320) antibody

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Anti PP1 Alpha (pThr320) Antibody gallery image 1 — Western blot analysis of extracts from HeLa cells untreated or treated with calyculin A (CA) using Rabbit anti PP1 ALPHA (pThr320) antibody. Cells were cultured in serum free media overnight before treatment with CA (100nM) for 30 minutes. 3% BSA was used for blocking

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Rabbit anti PP1 Alpha (pThr320)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: PP1 Alpha (pThr320)

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AHP2702 Datasheet Datasheet SDS SDS	WB	50 µl		Log in	Get Quote
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Rabbit anti PP1 alpha (pThr320) antibody recognizes serine/threonine-protein phosphatase PP1-alpha catalytic subunit, also known as PPP1A, when phosphorylated at threonine 320. PP1 alpha is one of three catalytic subunits of protein phosphatase 1 (PP1). Catalytic subunits can form complexes with regulatory proteins to form distinct multimeric holoenzymes (Ceulemans, H. & Bollen, M. 2004). PP1 activity is crucial for cell cycle progression. PP1 dephosphorylates many substrates target including retinoblastoma protein (Rb) (Durfee, T. et al. 1993). Phosphorylation of PP1 alpha on residue threonine 320 is performed by Cyclin-dependent kinase 1 (CDC2) kinase and leads to inhibition of its activity (Kwon, Y.G. el al. 1997).

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse Expected from Sequence; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to PP1 alpha (pThr320) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide
50% Glycerol
Immunogen: Phospho specific-peptide corresponding to residues surrounding threonine 320 of human PP1 alpha
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Mouse	Expected from Sequence

Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/500	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in	Get Quote
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Description	TidyBlot Western Blot Detection Reagent:HRP

Description	Product Code	Applications	Pack Size	Your Price
Mouse anti PP1 Alpha	VMA00742	WB	100 µl	Log in	Get Quote
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Description	Mouse anti PP1 Alpha

UniProt: P62136
Entrez Gene: PPP1CA
GO Terms: GO:0005515 protein binding; GO:0005829 cytosol; GO:0004722 protein serine/threonine phosphatase activity; GO:0046872 metal ion binding; GO:0005730 nucleolus; GO:0005977 glycogen metabolic process; GO:0006470 protein dephosphorylation; GO:0007049 cell cycle; GO:0008599 protein phosphatase type 1 regulator activity; View More GO Terms; GO:0019433 triglyceride catabolic process; GO:0051301 cell division; GO:0070688 MLL5-L complex; GO:0072357 PTW/PP1 phosphatase complex