PP1 Alpha (pThr320) antibody
Rabbit anti PP1 alpha (pThr320) antibody recognizes serine/threonine-protein phosphatase PP1-alpha catalytic subunit, also known as PPP1A, when phosphorylated at threonine 320. PP1 alpha is one of three catalytic subunits of protein phosphatase 1 (PP1). Catalytic subunits can form complexes with regulatory proteins to form distinct multimeric holoenzymes (Ceulemans, H. & Bollen, M. 2004). PP1 activity is crucial for cell cycle progression. PP1 dephosphorylates many substrates target including retinoblastoma protein (Rb) (Durfee, T. et al. 1993). Phosphorylation of PP1 alpha on residue threonine 320 is performed by Cyclin-dependent kinase 1 (CDC2) kinase and leads to inhibition of its activity (Kwon, Y.G. el al. 1997).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to PP1 alpha (pThr320) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding threonine 320 of human PP1 alpha
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P62136
- Entrez Gene
- PPP1CA
- GO Terms
- GO:0005515 protein binding
- GO:0005829 cytosol
- GO:0004722 protein serine/threonine phosphatase activity
- GO:0046872 metal ion binding
- GO:0005730 nucleolus
- GO:0005977 glycogen metabolic process
- GO:0006470 protein dephosphorylation
- GO:0007049 cell cycle
- GO:0008599 protein phosphatase type 1 regulator activity
- GO:0019433 triglyceride catabolic process
- GO:0051301 cell division
- GO:0070688 MLL5-L complex
- GO:0072357 PTW/PP1 phosphatase complex
- Regulatory
- For research purposes only
Applications of PP1 Alpha (pThr320) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti PP1 Alpha | VMA00742 | WB | 100 µl |
|
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
