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PP1 Alpha (pThr320) antibody

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Western blot analysis of extracts from HeLa cells untreated or treated with calyculin A (CA) using Rabbit anti PP1 ALPHA (pThr320) antibody. Cells were cultured in serum free media overnight before treatment with CA (100nM) for 30 minutes. 3% BSA was used for blocking

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Rabbit anti PP1 Alpha (pThr320)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
PP1 Alpha (pThr320)

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AHP2702
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Rabbit anti PP1 alpha (pThr320) antibody recognizes serine/threonine-protein phosphatase PP1-alpha catalytic subunit, also known as PPP1A, when phosphorylated at threonine 320. PP1 alpha is one of three catalytic subunits of protein phosphatase 1 (PP1). Catalytic subunits can form complexes with regulatory proteins to form distinct multimeric holoenzymes (Ceulemans, H. & Bollen, M. 2004). PP1 activity is crucial for cell cycle progression. PP1 dephosphorylates many substrates target including retinoblastoma protein (Rb) (Durfee, T. et al. 1993). Phosphorylation of PP1 alpha on residue threonine 320 is performed by Cyclin-dependent kinase 1 (CDC2) kinase and leads to inhibition of its activity (Kwon, Y.G. el al. 1997).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to PP1 alpha (pThr320) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding threonine 320 of human PP1 alpha
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
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Description TidyBlot Western Blot Detection Reagent:HRP

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Mouse anti PP1 Alpha VMA00742 WB 100 µl loader
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Description Mouse anti PP1 Alpha

UniProt
P62136
Entrez Gene
PPP1CA
GO Terms
GO:0005515 protein binding
GO:0005829 cytosol
GO:0004722 protein serine/threonine phosphatase activity
GO:0046872 metal ion binding
GO:0005730 nucleolus
GO:0005977 glycogen metabolic process
GO:0006470 protein dephosphorylation
GO:0007049 cell cycle
GO:0008599 protein phosphatase type 1 regulator activity
GO:0019433 triglyceride catabolic process
GO:0051301 cell division
GO:0070688 MLL5-L complex
GO:0072357 PTW/PP1 phosphatase complex
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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