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POU2F1 antibody

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Western Blot Validated
Anti POU2F1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with POU2F1 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 200 second exposure. Arrow points to POU2F1 (molecular weight 94 kDa)

Goat anti POU2F1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00130
Datasheet
WB
MSDS
100 µl

Goat anti Human POU2F1 antibody recognizes the POU domain, class 2, transcription factor 1, also known as NF-A1 Octamer-binding protein 1, Oct-1 Octamer-binding transcription factor 1 or OTF-1.

The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al. 1993 (PubMed 8314572)). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT (supplied by OMIM, Jul 2010).

Goat anti Human POU2F1 antibody detects a band of 94 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti POU2F1 detects a band of approximately 94 kDa in Ramos cell lysates.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5% BSA
Immunogen
Peptide with the sequence C-NVQSKSNEESGDSQQ, from the internal region of the protein sequence

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P14859
Entrez Gene
POU2F1
GO Terms
GO:0005515 protein binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0006383 transcription from RNA polymerase III promoter
GO:0016564 transcription repressor activity
GO:0043565 sequence-specific DNA binding
GO:0045892 negative regulation of transcription, DNA-dependent
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of POU2F1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
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