POU2F1 antibody

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Anti POU2F1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Goat anti Human POU2F1 antibody (**VPA00130**) followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 200 second exposure. Arrow points to POU2F1 (molecular weight 94 kDa).

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Goat anti POU2F1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: POU2F1

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VPA00130 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Goat anti Human POU2F1 antibody recognizes the POU domain, class 2, transcription factor 1, also known as NF-A1 Octamer-binding protein 1, Oct-1 Octamer-binding transcription factor 1 or OTF-1.

The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al. 1993 (PubMed 8314572)). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT (supplied by OMIM, Jul 2010).

Goat anti Human POU2F1 antibody detects a band of 94 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti POU2F1 detects a band of approximately 94 kDa in Ramos cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃) 0.5% BSA
Immunogen: Peptide with the sequence C-NVQSKSNEESGDSQQ, from the internal region of the protein sequence
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Donkey anti Sheep/Goat IgG:HRP	STAR88P	C E P WB	1 ml	Log in	Get Quote
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Description	Donkey anti Sheep/Goat IgG:HRP

UniProt: P14859
Entrez Gene: POU2F1
GO Terms: GO:0005515 protein binding; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0006383 transcription from RNA polymerase III promoter; GO:0016564 transcription repressor activity; GO:0043565 sequence-specific DNA binding; GO:0045892 negative regulation of transcription, DNA-dependent