POU2F1 antibody
Goat anti POU2F1
POU2F1 antibody recognizes POU domain, class 2, transcription factor 1, also known as NF-A1, OTF-1, Octamer-binding transcription factor-1,Oct1, octamer-binding protein 1 or octamer-binding transcription factor 1.
POU2F1 Antibody with Control Lysate
POU2F1 antibody recognizes POU domain, class 2, transcription factor 1, also known as NF-A1, OTF-1, Octamer-binding transcription factor-1,Oct1, octamer-binding protein 1 or octamer-binding transcription factor 1.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00130 | WB | 100 µl | ||||
| VPA00130KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human POU2F1 antibody recognizes the POU domain, class 2, transcription factor 1, also known as NF-A1 Octamer-binding protein 1, Oct-1 Octamer-binding transcription factor 1 or OTF-1.
The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al. 1993 (PubMed 8314572)). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT (supplied by OMIM, Jul 2010).
Goat anti Human POU2F1 antibody detects a band of 94 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al. 1993 (PubMed 8314572)). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT (supplied by OMIM, Jul 2010).
Goat anti Human POU2F1 antibody detects a band of 94 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti POU2F1 detects a band of approximately 94 kDa in Ramos cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography.
- Preparation
- 20μl Goat polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3) 0.5% BSA
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3) 0.5% BSA
- Immunogen
- Peptide with the sequence C-NVQSKSNEESGDSQQ, from the internal region of the protein sequence
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Ramos lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P14859 Related reagents
- Entrez Gene
- POU2F1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0006383 transcription from RNA polymerase III promoter
- GO:0016564 transcription repressor activity
- GO:0043565 sequence-specific DNA binding
- GO:0045892 negative regulation of transcription, DNA-dependent
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of POU2F1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
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