Polyadenylate-Binding Protein 1 antibody
Rabbit anti Polyadenylate-Binding Protein 1
Polyadenylate-binding protein 1 antibody recognizes polyadenylate-binding protein 1, also known as PABPC1 or poly(A) binding protein, cytoplasmic 2. This antibody has been extensively validated for western blotting using whole cell lysates.
Polyadenylate-Binding Protein 1 Antibody with Control Lysate
Polyadenylate-binding protein 1 antibody recognizes polyadenylate-binding protein 1, also known as PABPC1 or poly(A) binding protein, cytoplasmic 2. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00225 | WB | 100 µl | ||||
| VPA00225KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human polyadenylate-binding protein 1 antibody recognizes polyadenylate-binding protein 1, also known as poly(A) binding protein, cytoplasmic 2.
Encoded by the PABPC1 gene, polyadenylate-binding protein 1 is a poly(A) binding protein. This protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes (provided by RefSeq, Aug 2010).
Rabbit anti Human polyadenylate-binding protein 1 antibody detects a band of 71 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the PABPC1 gene, polyadenylate-binding protein 1 is a poly(A) binding protein. This protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes (provided by RefSeq, Aug 2010).
Rabbit anti Human polyadenylate-binding protein 1 antibody detects a band of 71 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti polyadenylate-binding protein 1 detects a band of approximately 71 kDa in HeLa cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Preparation
- 20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human polyadenylate-binding protein 1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P11940 Related reagents
- Entrez Gene
- PABPC1 Related reagents
- GO Terms
- GO:0000166 nucleotide binding
- GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening
- GO:0005829 cytosol
- GO:0005681 spliceosomal complex
- GO:0006378 mRNA polyadenylation
- GO:0008143 poly(A) RNA binding
- GO:0006412 translation
- GO:0008022 protein C-terminus binding
- GO:0008380 RNA splicing
- GO:0008494 translation activator activity
- GO:0048255 mRNA stabilization
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Polyadenylate-Binding Protein 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies
Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.
Contact Information
Online Payment Methods:
- Invoice / Purchase Order
- Credit Card (Maestro, VISA,MasterCard & VISA Electron)
