Polyadenylate-Binding Protein 1 antibody

Rabbit anti Polyadenylate-Binding Protein 1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Polyadenylate-Binding Protein 1

Product Code Applications Pack Size List Price Your Price Qty
VPA00225
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Rabbit anti Human polyadenylate-binding protein 1 antibody recognizes polyadenylate-binding protein 1, also known as poly(A) binding protein, cytoplasmic 2.

Encoded by the PABPC1 gene, polyadenylate-binding protein 1 is a poly(A) binding protein. This protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes (provided by RefSeq, Aug 2010).

Rabbit anti Human polyadenylate-binding protein 1 antibody detects a band of 71 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti polyadenylate-binding protein 1 detects a band of approximately 71 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human polyadenylate-binding protein 1
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P11940
Entrez Gene
PABPC1
GO Terms
GO:0000166 nucleotide binding
GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening
GO:0005829 cytosol
GO:0005681 spliceosomal complex
GO:0006378 mRNA polyadenylation
GO:0008143 poly(A) RNA binding
GO:0006412 translation
GO:0008022 protein C-terminus binding
GO:0008380 RNA splicing
GO:0008494 translation activator activity
GO:0048255 mRNA stabilization
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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