Polyadenylate-Binding Protein 1 antibody

100% Secure


Rabbit anti Polyadenylate-Binding Protein 1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00225
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human polyadenylate-binding protein 1 antibody recognizes polyadenylate-binding protein 1, also known as poly(A) binding protein, cytoplasmic 2.

Encoded by the PABPC1 gene, polyadenylate-binding protein 1 is a poly(A) binding protein. This protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes (provided by RefSeq, Aug 2010).

Rabbit anti Human polyadenylate-binding protein 1 antibody detects a band of 71 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti polyadenylate-binding protein 1 detects a band of approximately 71 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human polyadenylate-binding protein 1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P11940 Related reagents
Entrez Gene
PABPC1 Related reagents
GO Terms
GO:0000166 nucleotide binding
GO:0000289 nuclear-transcribed mRNA poly(A) tail shortening
GO:0005829 cytosol
GO:0005681 spliceosomal complex
GO:0006378 mRNA polyadenylation
GO:0008143 poly(A) RNA binding
GO:0006412 translation
GO:0008022 protein C-terminus binding
GO:0008380 RNA splicing
GO:0008494 translation activator activity
GO:0048255 mRNA stabilization
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Polyadenylate-Binding Protein 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml

Western Blotting without Trial and Error: Use PrecisionAb Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.