Polyadenylate-Binding Nuclear Protein 1 antibody

Rabbit anti Polyadenylate-Binding Nuclear Protein 1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Polyadenylate-Binding Nuclear Protein 1

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VPA00915
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Rabbit anti Human polyadenylate-binding nuclear protein 1 antibody recognizes polyadenylate-binding protein 1, also known as poly(A) binding protein 1, OPMD and PABPN1.

PABPN1 encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. PABPN1 contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between PABPN1 and the neighboring upstream BCL2-like 2 (BCL2L2) gene (provided by RefSeq, Dec 2010).

Rabbit anti Human polyadenylate-binding protein 1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti polyadenylate-binding nuclear protein 1 antibody recognizes a band of approximately 50 kDa in Raji cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
<50% Glycerol
Immunogen
Synthetic peptide of human polyadenylate-binding nuclear protein 1
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
Q86U42
Entrez Gene
PABPN1
GO Terms
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0003723 RNA binding
GO:0030529 ribonucleoprotein complex
GO:0006936 muscle contraction
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0006369 termination of RNA polymerase II transcription
GO:0016973 poly(A)+ mRNA export from nucleus
GO:0019058 viral infectious cycle
GO:0031124 mRNA 3'-end processing
GO:0046778 modification by virus of host mRNA processing
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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