Polyadenylate-Binding Nuclear Protein 1 antibody

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Anti Polyadenylate-Binding Nuclear Protein 1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with polyadenylate-binding nuclear protein 1 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 1 second exposure. Arrow points to polyadenylate-binding nuclear protein 1 (molecular weight 50 kDa)

Rabbit anti Polyadenylate-Binding Nuclear Protein 1

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Polyadenylate-Binding Nuclear Protein 1

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Rabbit anti Human polyadenylate-binding nuclear protein 1 antibody recognizes polyadenylate-binding protein 1, also known as poly(A) binding protein 1, OPMD and PABPN1.

PABPN1 encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails at the 3' ends of eukaryotic transcripts and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. PABPN1 contains a GCG trinucleotide repeat at the 5' end of the coding region, and expansion of this repeat from the normal 6 copies to 8-13 copies leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Related pseudogenes have been identified on chromosomes 19 and X. Read-through transcription also exists between PABPN1 and the neighboring upstream BCL2-like 2 (BCL2L2) gene (provided by RefSeq, Dec 2010).

Rabbit anti Human polyadenylate-binding protein 1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti polyadenylate-binding nuclear protein 1 antibody recognizes a band of approximately 50 kDa in Raji cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography on immunogen
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
<50% Glycerol
Immunogen: Synthetic peptide of human polyadenylate-binding nuclear protein 1
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: Q86U42
Entrez Gene: PABPN1
GO Terms: GO:0000166 nucleotide binding; GO:0005515 protein binding; GO:0000398 nuclear mRNA splicing, via spliceosome; GO:0003723 RNA binding; GO:0030529 ribonucleoprotein complex; GO:0006936 muscle contraction; GO:0005737 cytoplasm; GO:0005654 nucleoplasm; GO:0006369 termination of RNA polymerase II transcription; View More GO Terms; GO:0016973 poly(A)+ mRNA export from nucleus; GO:0019058 viral infectious cycle; GO:0031124 mRNA 3'-end processing; GO:0046778 modification by virus of host mRNA processing