Poly [ADP-RIBOSE] Polymerase 3 antibody

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Rabbit anti Poly (ADP-Ribose) Polymerase 3

Rabbit anti-human poly (ADP-ribose) polymerase 3 antibody recognizes poly (ADP-ribose) polymerase 3, also known as ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 2, ADP-ribosyltransferase diphtheria toxin-like 3 or ADPRT-3.

Poly [ADP-RIBOSE] Polymerase 3 Antibody with Control Lysate

Rabbit anti-human poly (ADP-ribose) polymerase 3 antibody recognizes poly (ADP-ribose) polymerase 3, also known as ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 2, ADP-ribosyltransferase diphtheria toxin-like 3 or ADPRT-3.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00523 WB 100 µl
VPA00523KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human poly [ADP-ribose] polymerase 3 antibody recognizes poly [ADP-ribose] polymerase 3 also known as PARP3, ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 3, ADP-ribosyltransferase diphtheria toxin-like 3, NAD(+) ADP-ribosyltransferase 3, IRT1 or poly[ADP-ribose] synthase 3.

The protein encoded by the PARP3 gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. PARP3 gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified (provided by RefSeq, Jul 2008).

Rabbit anti Human poly [ADP-ribose] polymerase 3 antibody detects a band of 71 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti poly [adp-ribose] polymerase 3 detects a band of approximately 71 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid 99-126 of human poly [ADP-ribose] polymerase 3
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9Y6F1 Related reagents
Entrez Gene
PARP3 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003950 NAD+ ADP-ribosyltransferase activity
GO:0005634 nucleus
GO:0005814 centriole
GO:0006281 DNA repair
GO:0006471 protein ADP-ribosylation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Poly [ADP-RIBOSE] Polymerase 3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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