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Poly (ADP-Ribose) Polymerase-1 antibody

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Anti Poly (ADP-Ribose) Polymerase-1 Antibody gallery image 1
Western blot analysis of THP-1 cell lysate either untreated or treated with TNF-alpha and cycloheximide (CHX) using Rabbit anti Poly (ADP-Ribose) Polymerase-1 antibody. 3% non-fat dry milk was used for blocking

Rabbit anti Poly (ADP-Ribose) Polymerase-1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2727
Datasheet
WB
SDS
50 µl loader

Rabbit anti poly (ADP-ribose) polymerase-1 antibody recognizes Poly (ADP-Ribose) Polymerase-1, also known as PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1 (ARTD1), NAD(+) ADP-ribosyltransferase 1 (ADPRT 1) and Poly[ADP-ribose] synthase 1. It belongs to the Poly(ADP-ribose) polymerase (PARP) family. The PARP family is composed of 18 proteins, encoded by different genes and displaying a conserved catalytic domain (Dantzer at al. 2004). PARP1 is involved in DNA repair (Rouleau et al. 2010), chromatin remodeling, transcription, replication and recombination (Morales et al. 2014). Cleavage of PARP1 has been used extensively as a marker of apoptosis (Oliver et al. 1998).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to poly (ADP-ribose) polymerase-1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
A synthetic peptide of human poly (ADP-ribose) polymerase-1

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P09874
Entrez Gene
PARP1
GO Terms
GO:0003677 DNA binding
GO:0003950 NAD+ ADP-ribosyltransferase activity
GO:0005635 nuclear envelope
GO:0005667 transcription factor complex
GO:0005730 nucleolus
GO:0006366 transcription from RNA polymerase II promoter
GO:0006471 protein ADP-ribosylation
GO:0008134 transcription factor binding
GO:0008270 zinc ion binding
GO:0042802 identical protein binding
GO:0032869 cellular response to insulin stimulus
GO:0045449 regulation of transcription
GO:0047485 protein N-terminus binding
Regulatory
For research purposes only

Applications of Poly (ADP-Ribose) Polymerase-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti poly (ADP-ribose) polymerase-1 antibody detects a band of approximately 113 kDa (full length) and 90 kDa (cleaved) in THP-1 cell lysate under reducing conditions
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
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