Poly (ADP-Ribose) Polymerase-1 antibody
Rabbit anti poly (ADP-ribose) polymerase-1 antibody recognizes Poly (ADP-Ribose) Polymerase-1, also known as PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1 (ARTD1), NAD(+) ADP-ribosyltransferase 1 (ADPRT 1) and Poly[ADP-ribose] synthase 1. It belongs to the Poly(ADP-ribose) polymerase (PARP) family. The PARP family is composed of 18 proteins, encoded by different genes and displaying a conserved catalytic domain (Dantzer at al. 2004). PARP1 is involved in DNA repair (Rouleau et al. 2010), chromatin remodeling, transcription, replication and recombination (Morales et al. 2014). Cleavage of PARP1 has been used extensively as a marker of apoptosis (Oliver et al. 1998).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to poly (ADP-ribose) polymerase-1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- A synthetic peptide of human poly (ADP-ribose) polymerase-1
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P09874
- Entrez Gene
- PARP1
- GO Terms
- GO:0003677 DNA binding
- GO:0003950 NAD+ ADP-ribosyltransferase activity
- GO:0005635 nuclear envelope
- GO:0005667 transcription factor complex
- GO:0005730 nucleolus
- GO:0006366 transcription from RNA polymerase II promoter
- GO:0006471 protein ADP-ribosylation
- GO:0008134 transcription factor binding
- GO:0008270 zinc ion binding
- GO:0042802 identical protein binding
- GO:0032869 cellular response to insulin stimulus
- GO:0045449 regulation of transcription
- GO:0047485 protein N-terminus binding
- Regulatory
- For research purposes only
Applications of Poly (ADP-Ribose) Polymerase-1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti poly (ADP-ribose) polymerase-1 antibody detects a band of approximately 113 kDa (full length) and 90 kDa (cleaved) in THP-1 cell lysate under reducing conditions
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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