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POLR2H antibody

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Anti POLR2H Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti POLR2H Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2
Anti POLR2H Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with POLR2H antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 20 second exposure. Arrow points to POLR2H (molecular weight 17 kDa)
Anti POLR2H Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Immunoprecipitation of POLR2H from Jurkat whole cell lysate. The Input lane is 10% input; the POLR2H-IP lane is the immunoprecipitation with the POLR2H antibody (2.5 µg, VPA00356); the IgG-IP lane is the immunoprecipitation with Rabbit polyclonal IgG(2.5 µg, PRABP01). Western blot analysis was performed using POLR2H antibody (1/1,000, VPA00356) followed by detection with HRP conjugated TidyBlot Western Blot Detection Reagent (1/250, STAR209) and visualized on the ChemiDoc MP. Arrow points to POLR2H.

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WB IP

Rabbit anti POLR2H

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
POLR2H

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Product Code Applications Pack Size List Price Your Price Qty
VPA00356
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SDS Safety Datasheet SDS
IP WB 100 µl loader
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loader

Rabbit anti Human POLR2H antibody recognizes POLRH2, also known as RPB8, RPB17, DNA-directed RNA polymerase II subunit H, DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide and DNA-directed RNA polymerases I, II, and III subunit RPABC3.

The three eukaryotic RNA polymerases are complex multi-subunit enzymes that play a central role in the transcription of nuclear genes. The POLR2H gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of POLR2H (provided by RefSeq, Jul 2013).

Rabbit anti Human POLR2H antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti POLR2H detects a band of approximately 17 kDa in Jurkat cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 59-87 of human POLR2H
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml loader
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loader
Description Goat anti Rabbit IgG (H/L):HRP

UniProt
P52434
Entrez Gene
POLR2H
GO Terms
GO:0005515 protein binding
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0003899 DNA-directed RNA polymerase activity
GO:0005665 DNA-directed RNA polymerase II, core complex
GO:0005730 nucleolus
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006361 transcription initiation from RNA polymerase I promoter
GO:0006362 transcription elongation from RNA polymerase I promoter
GO:0006363 termination of RNA polymerase I transcription
GO:0006367 transcription initiation from RNA polymerase II promoter
GO:0006368 transcription elongation from RNA polymerase II promoter
GO:0006370 mRNA capping
GO:0006385 transcription elongation from RNA polymerase III promoter
GO:0006386 termination of RNA polymerase III transcription
GO:0008270 zinc ion binding
GO:0016032 viral reproduction
GO:0050434 positive regulation of viral transcription

VPA00356

160309

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