POLB antibody

Rabbit anti POLB

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
POLB

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VPA00190
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Rabbit anti Human POLB antibody recognizes POLB, also known as DNA pol beta, DNA polymerase beta or DNA polymerase beta subunit.

The protein encoded by the POLB gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. POLB acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of POLB exist, but the full-length nature of only one has been described to date (provided by RefSeq, Sep 2011).

Rabbit anti Human POLB antibody detects a band of 38 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti POLB detects a band of approximately 38 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to a region near the C-terminus of the POLB protein
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P06746
Entrez Gene
POLB
GO Terms
GO:0003887 DNA-directed DNA polymerase activity
GO:0003906 DNA-(apurinic or apyrimidinic site) lyase activity
GO:0046872 metal ion binding
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0005876 spindle microtubule
GO:0006261 DNA-dependent DNA replication
GO:0008017 microtubule binding
GO:0019899 enzyme binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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