Pokemon antibody

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Rabbit anti Pokemon

Pokemon Antibody with Control Lysate

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00636 WB 100 µl
VPA00636KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human pokemon antibody recognizes the zinc finger and BTB domain-containing protein 7A, also known as HIV-1 inducer of short transcripts binding protein, POZ and Krueppel erythroid myeloid ontogenic factor, TTF-I-interacting peptide 21 (TIP21), LRF or zinc finger protein 857A.

Based on its similarity to other proteins Zinc finger and BTB domain-containing, the protein encoded by ZBTB7A is believed to play a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals and/or to efficiently abrogate E2F1-dependent CDKN2A transactivation/de-repression. Expressed in medullary epithelial cells and Hassle's corpuscles in WT thymus and in the squamous epithelium as well as the germinal center lymphocytes in tonsils. The product of ZBTB7A is up-regulated in a subset of lymphomas, as well as in a subset of breast, lung, colon, prostate and bladder carcinomas (at protein level). ZBTB7A has been shown to interact with BCL6.

Rabbit anti Human pokemon antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti pokemon detects a band of approximately 75 kDa in HEK293 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Immunogen
Synthetic peptide encompassing part of the N terminal region of human pokemon
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
O95365 Related reagents
Entrez Gene
ZBTB7A Related reagents
GO Terms
GO:0003677 DNA binding
GO:0005634 nucleus
GO:0006350 transcription
GO:0007275 multicellular organismal development
GO:0008270 zinc ion binding
GO:0016564 transcription repressor activity
GO:0030154 cell differentiation
GO:0035035 histone acetyltransferase binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Pokemon antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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