Pokemon antibody
Rabbit anti Human pokemon antibody recognizes the zinc finger and BTB domain-containing protein 7A, also known as HIV-1 inducer of short transcripts binding protein, POZ and Krueppel erythroid myeloid ontogenic factor, TTF-I-interacting peptide 21 (TIP21), LRF or zinc finger protein 857A.
Based on its similarity to other proteins Zinc finger and BTB domain-containing, the protein encoded by ZBTB7A is believed to play a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals and/or to efficiently abrogate E2F1-dependent CDKN2A transactivation/de-repression. Expressed in medullary epithelial cells and Hassle's corpuscles in WT thymus and in the squamous epithelium as well as the germinal center lymphocytes in tonsils. The product of ZBTB7A is up-regulated in a subset of lymphomas, as well as in a subset of breast, lung, colon, prostate and bladder carcinomas (at protein level). ZBTB7A has been shown to interact with BCL6.
Rabbit anti Human pokemon antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Based on its similarity to other proteins Zinc finger and BTB domain-containing, the protein encoded by ZBTB7A is believed to play a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals and/or to efficiently abrogate E2F1-dependent CDKN2A transactivation/de-repression. Expressed in medullary epithelial cells and Hassle's corpuscles in WT thymus and in the squamous epithelium as well as the germinal center lymphocytes in tonsils. The product of ZBTB7A is up-regulated in a subset of lymphomas, as well as in a subset of breast, lung, colon, prostate and bladder carcinomas (at protein level). ZBTB7A has been shown to interact with BCL6.
Rabbit anti Human pokemon antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti pokemon detects a band of approximately 75 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Preservative Stabilisers
- 0.09% Sodium Azide
2% Sucrose - Immunogen
- Synthetic peptide encompassing part of the N terminal region of human pokemon
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- O95365 Related reagents
- Entrez Gene
- ZBTB7A Related reagents
- GO Terms
- GO:0003677 DNA binding
- GO:0005634 nucleus
- GO:0006350 transcription
- GO:0007275 multicellular organismal development
- GO:0008270 zinc ion binding
- GO:0016564 transcription repressor activity
- GO:0030154 cell differentiation
- GO:0035035 histone acetyltransferase binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Pokemon antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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