Plasminogen antibody

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Sheep anti Human Plasminogen:FITC

Sheep anti Human Plasminogen:HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
7440-0104F IF 1 ml
7440-0104P C E 1 ml
Sheep anti Human plasminogen antibody recognizes human plasminogen, a ~90 kDa single-chain glycoprotein and inactive precursor (zymogen) of plasmin, secreted by the liver and present in the blood and majority of extravascular body fluids.

Plasminogen is the central component in the fibrinolytic system, and is converted into active plasmin through cleavage by the endogenous activators urokinase (u-PA) and tissue plasminogen activator (t-PA), in the presence of fibrin. The level of plasminogen is maintained in the plasma at around 200mg/L, with an increase being associated with hypothyroidism, obesity and pregnancy, and deficiencies with many disorders including thrombosis, liver disease, sepsis and hyperthyroidism.

Sheep anti Human plasminogen antibody has not been cross adsorbed and may cross react with plasminogen from other species.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cat
Rat
Dog
Bovine
Guinea Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to human plasminogen were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.01%Thiomersal
Immunogen
Human plasminogen, purified from plasma.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P00747 Related reagents
Entrez Gene
PLG Related reagents
GO Terms
GO:0002576 platelet degranulation
GO:0004252 serine-type endopeptidase activity
GO:0005615 extracellular space
GO:0008285 negative regulation of cell proliferation
GO:0010812 negative regulation of cell-substrate adhesion
GO:0022617 extracellular matrix disassembly
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0031232 extrinsic to external side of plasma membrane
GO:0034185 apolipoprotein binding
GO:0042730 fibrinolysis
GO:0043498 cell surface binding
GO:0048771 tissue remodeling
GO:0051918 negative regulation of fibrinolysis
GO:0051919 positive regulation of fibrinolysis
Regulatory
For research purposes only

Applications of Plasminogen antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
ELISA
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for Plasminogen antibody

  1. Clinton, S.R. et al. (2010) Binding and activation of host plasminogen on the surface of Francisella tularensis.
    BMC Microbiol. 10:76.
  2. Samson, A.L. et al. (2009) A nonfibrin macromolecular cofactor for tPA-mediated plasmin generation following cellular injury.
    Blood. 114: 1937-46.
  3. Samson, A.L. et al. (2008) Tissue-type plasminogen activator requires a co-receptor to enhance NMDA receptor function.
    J Neurochem. 107: 1091-101.
  4. Singh, B. et al. (2015) Moraxella catarrhalis Binds Plasminogen To Evade Host Innate Immunity.
    Infect Immun. 83 (9): 3458-69.
  5. Borg, R.J. et al. (2015) Dendritic Cell-Mediated Phagocytosis but Not Immune Activation Is Enhanced by Plasmin.
    PLoS One. 10 (7): e0131216.

Further Reading

  1. Fears, R. (1989) Binding of plasminogen activators to fibrin: characterization and pharmacological consequences.
    Biochem J. 261 (2): 313-24.