PKA C-ALPHA antibody
Rabbit anti PKA C-alpha antibody recognizes cAMP-dependent protein kinase catalytic subunit alpha, also known as PRKACA. The function and specificity of PKA C-alpha depend on subcellular localization, expression of isoforms and physio-chemical features (Søberg et al. 2013, Skalhegg and Tasken 2000). It phosphorylates a large number of proteins, for example, p50 at serine 337 (Guan et al. 2005), claudin-4 at threonine 192 (D'Souza et al. 2005). Dnajb1–Prkaca gene fusion drives tumorigenesis in mice (Kastenhuber et al. 2017).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to PKA C-alpha was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- A synthetic peptide corresponding to amino acids 9-40 of human PKA C-alpha
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P17612
- Entrez Gene
- PRKACA
- GO Terms
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0007596 blood coagulation
- GO:0005829 cytosol
- GO:0004691 cAMP-dependent protein kinase activity
- GO:0004862 cAMP-dependent protein kinase inhibitor activity
- GO:0005524 ATP binding
- GO:0005654 nucleoplasm
- GO:0005952 cAMP-dependent protein kinase complex
- GO:0006094 gluconeogenesis
- GO:0006112 energy reserve metabolic process
- GO:0006833 water transport
- GO:0007202 activation of phospholipase C activity
- GO:0007243 intracellular protein kinase cascade
- GO:0050796 regulation of insulin secretion
- GO:0019433 triglyceride catabolic process
- GO:0019901 protein kinase binding
- GO:0034199 activation of protein kinase A activity
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0055085 transmembrane transport
- GO:0071377 cellular response to glucagon stimulus
- Regulatory
- For research purposes only
Applications of PKA C-ALPHA antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti PKA C-alpha antibody detects a band of approximately 41 kDa in HeLa cell lysate under reducing conditions
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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