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PKA C-ALPHA antibody

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Western blot analysis of HeLa cell lysate using Rabbit anti PKA C-alpha antibody. 3% non-fat dry milk was used for blocking

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Rabbit anti PKA C-ALPHA

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
PKA C-ALPHA

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
AHP2719
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SDS Safety Datasheet SDS
WB 50 µl loader
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Rabbit anti PKA C-alpha antibody recognizes cAMP-dependent protein kinase catalytic subunit alpha, also known as PRKACA. The function and specificity of PKA C-alpha depend on subcellular localization, expression of isoforms and physio-chemical features (Søberg et al. 2013, Skalhegg and Tasken 2000). It phosphorylates a large number of proteins, for example, p50 at serine 337 (Guan et al. 2005), claudin-4 at threonine 192 (D'Souza et al. 2005). Dnajb1–Prkaca gene fusion drives tumorigenesis in mice (Kastenhuber et al. 2017).

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to PKA C-alpha was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
A synthetic peptide corresponding to amino acids 9-40 of human PKA C-alpha
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti PKA C-alpha antibody detects a band of approximately 41 kDa in HeLa cell lysate under reducing conditions

Description Product Code Applications Pack Size List Price Your Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

UniProt
P17612
Entrez Gene
PRKACA
GO Terms
GO:0000086 G2/M transition of mitotic cell cycle
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0004691 cAMP-dependent protein kinase activity
GO:0004862 cAMP-dependent protein kinase inhibitor activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0005952 cAMP-dependent protein kinase complex
GO:0006094 gluconeogenesis
GO:0006112 energy reserve metabolic process
GO:0006833 water transport
GO:0007202 activation of phospholipase C activity
GO:0007243 intracellular protein kinase cascade
GO:0050796 regulation of insulin secretion
GO:0019433 triglyceride catabolic process
GO:0019901 protein kinase binding
GO:0034199 activation of protein kinase A activity
GO:0048011 nerve growth factor receptor signaling pathway
GO:0055085 transmembrane transport
GO:0071377 cellular response to glucagon stimulus
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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