PKA C-ALPHA antibody

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Anti PKA C-ALPHA Antibody gallery image 1 — Western blot analysis of HeLa cell lysate using Rabbit anti PKA C-alpha antibody. 3% non-fat dry milk was used for blocking

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Rabbit anti PKA C-ALPHA

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: PKA C-ALPHA

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Rabbit anti PKA C-alpha antibody recognizes cAMP-dependent protein kinase catalytic subunit alpha, also known as PRKACA. The function and specificity of PKA C-alpha depend on subcellular localization, expression of isoforms and physio-chemical features (Søberg et al. 2013, Skalhegg and Tasken 2000). It phosphorylates a large number of proteins, for example, p50 at serine 337 (Guan et al. 2005), claudin-4 at threonine 192 (D'Souza et al. 2005). Dnajb1–Prkaca gene fusion drives tumorigenesis in mice (Kastenhuber et al. 2017).

Target Species: Human
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to PKA C-alpha was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
Immunogen: A synthetic peptide corresponding to amino acids 9-40 of human PKA C-alpha
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/500	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: Rabbit anti PKA C-alpha antibody detects a band of approximately 41 kDa in HeLa cell lysate under reducing conditions

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TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in	Get Quote
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Description	TidyBlot Western Blot Detection Reagent:HRP

UniProt: P17612
Entrez Gene: PRKACA
GO Terms: GO:0000086 G2/M transition of mitotic cell cycle; GO:0007596 blood coagulation; GO:0005829 cytosol; GO:0004691 cAMP-dependent protein kinase activity; GO:0004862 cAMP-dependent protein kinase inhibitor activity; GO:0005524 ATP binding; GO:0005654 nucleoplasm; GO:0005952 cAMP-dependent protein kinase complex; GO:0006094 gluconeogenesis; View More GO Terms; GO:0006112 energy reserve metabolic process; GO:0006833 water transport; GO:0007202 activation of phospholipase C activity; GO:0007243 intracellular protein kinase cascade; GO:0050796 regulation of insulin secretion; GO:0019433 triglyceride catabolic process; GO:0019901 protein kinase binding; GO:0034199 activation of protein kinase A activity; GO:0048011 nerve growth factor receptor signaling pathway; GO:0055085 transmembrane transport; GO:0071377 cellular response to glucagon stimulus