PIAS3 antibody

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Rabbit anti PIAS3

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00570
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human PIAS3 antibody recognizes PIAS3, also known as E3 SUMO-protein ligase PIAS3, protein inhibitor of activated STAT protein 3 or "zinc finger, MIZ-type containing 5".

The PIAS3 gene encodes a member of the PIAS (protein inhibitor of activated STAT (signal transducer and activator of transcription) family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of PIAS3 have been identified, but the full-length nature of some of these variants has not been determined (provided by RefSeq, Jul 2008).

Rabbit anti Human PIAS3 antibody detects a band of 75 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PIAS3 detects a band of approximately 75 kDa in MCF7 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid 95-126 of human PIAS3

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9Y6X2
Entrez Gene
PIAS3
GO Terms
GO:0003676 nucleic acid binding
GO:0006350 transcription
GO:0008022 protein C-terminus binding
GO:0008270 zinc ion binding
GO:0016607 nuclear speck
GO:0016925 protein sumoylation
GO:0019899 enzyme binding
GO:0033235 positive regulation of protein sumoylation
GO:0045449 regulation of transcription
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of PIAS3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml