PI-4 Kinase 2A antibody
Rabbit anti PI-4 Kinase 2A
Rabbit anti human PI4K2A antibody recognizes phosphatidylinositol 4-kinase type 2-alpha, also known as phosphatidylinositol 4-kinase type II (PI4KII) or phosphatidylinositol 4-kinase type 2A. This antibody has been extensively validated for western blotting using whole cell lysates.
PI-4 Kinase 2A Antibody with Control Lysate
Rabbit anti human PI4K2A antibody recognizes phosphatidylinositol 4-kinase type 2-alpha, also known as phosphatidylinositol 4-kinase type II (PI4KII) or phosphatidylinositol 4-kinase type 2A. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00327 | WB | 100 µl | ||||
| VPA00327KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human PI-4 kinase 2A antibody recognizes PI-4 kinase 2A, also known as phosphatidylinositol 4-kinase type II (PI4KII), phosphatidylinositol 4-kinase type 2A and phosphatidylinositol 4-kinase type II-alpha.
Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI-4 Kinase 2A phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al. 2001 PubMed 11244087, OMIM, Mar 2008).
Rabbit anti Human PI-4 kinase 2A antibody detects a band of 54 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI-4 Kinase 2A phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al. 2001 PubMed 11244087, OMIM, Mar 2008).
Rabbit anti Human PI-4 kinase 2A antibody detects a band of 54 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti PI-4 kinase 2A detects a band of approximately 54 kDa in Jurkat cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- KLH-conjugated synthetic peptide corresponding to aa 387-415 of human PI-4 kinase 2A
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Guarantee
- As supplied, 12 months from date of despatch
- Guarantee
- As supplied, 12 months from date of despatch
More Information
- UniProt
- Q9BTU6 Related reagents
- Entrez Gene
- PI4K2A Related reagents
- GO Terms
- GO:0000287 magnesium ion binding
- GO:0004430 1-phosphatidylinositol 4-kinase activity
- GO:0005887 integral to plasma membrane
- GO:0005524 ATP binding
- GO:0005737 cytoplasm
- GO:0006661 phosphatidylinositol biosynthetic process
- GO:0045121 membrane raft
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of PI-4 Kinase 2A antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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