PI-4 Kinase 2A antibody

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Rabbit anti PI-4 Kinase 2A

Rabbit anti human PI4K2A antibody recognizes phosphatidylinositol 4-kinase type 2-alpha, also known as phosphatidylinositol 4-kinase type II (PI4KII) or phosphatidylinositol 4-kinase type 2A. This antibody has been extensively validated for western blotting using whole cell lysates.

PI-4 Kinase 2A Antibody with Control Lysate

Rabbit anti human PI4K2A antibody recognizes phosphatidylinositol 4-kinase type 2-alpha, also known as phosphatidylinositol 4-kinase type II (PI4KII) or phosphatidylinositol 4-kinase type 2A. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00327 WB 100 µl
VPA00327KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human PI-4 kinase 2A antibody recognizes PI-4 kinase 2A, also known as phosphatidylinositol 4-kinase type II (PI4KII), phosphatidylinositol 4-kinase type 2A and phosphatidylinositol 4-kinase type II-alpha.

Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI-4 Kinase 2A phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al. 2001 PubMed 11244087, OMIM, Mar 2008).

Rabbit anti Human PI-4 kinase 2A antibody detects a band of 54 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 387-415 of human PI-4 kinase 2A

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q9BTU6 Related reagents
Entrez Gene
PI4K2A Related reagents
GO Terms
GO:0000287 magnesium ion binding
GO:0004430 1-phosphatidylinositol 4-kinase activity
GO:0005887 integral to plasma membrane
GO:0005524 ATP binding
GO:0005737 cytoplasm
GO:0006661 phosphatidylinositol biosynthetic process
GO:0045121 membrane raft
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PI-4 Kinase 2A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti PI-4 kinase 2A detects a band of approximately 54 kDa in Jurkat cell lysate
Western Blotting
Anti PI-4 kinase 2A detects a band of approximately 54 kDa in Jurkat cell lysate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB