Rabbit anti PI-3 kinase p85 subunit alpha antibody recognizes PI-3 kinase p85 subunit alpha. PI-3 kinase p85 subunit alpha is a regulatory subunit of PI 3-kinase family of enzymes. PI 3-kinases are involved in a variety of cellular functions as part of the PI3K/AKT/mTOR pathway.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to PI-3 kinase p85 subunit alpha was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Recombinant human PI-3 kinase p85 subunit alpha
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
ErbB-3 class receptor binding
insulin receptor binding
insulin-like growth factor receptor binding
1-phosphatidylinositol-4-phosphate 3-kinase, class IA complex
epidermal growth factor receptor signaling pathway
insulin receptor signaling pathway
fibroblast growth factor receptor signaling pathway
phosphatidylinositol 3-kinase cascade
positive regulation of glucose import
protein phosphatase binding
T cell costimulation
phosphatidylinositol 3-kinase regulator activity
insulin receptor substrate binding
interspecies interaction between organisms
insulin-like growth factor receptor signaling pathway
nerve growth factor receptor signaling pathway
T cell receptor signaling pathway
growth hormone receptor signaling pathway
positive regulation of establishment of protein localization in plasma membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of PI-3 Kinase p85 Subunit Alpha antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti PI-3 kinase p85 subunit alpha antibody detects a band of approximately 85 kDa in THP-1 cell lysate under reducing conditions
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Secondary Antibodies Available
Useful Reagents Available