PI-3 Kinase p85 Subunit Alpha antibody

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Rabbit anti PI-3 Kinase p85 Subunit Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2508 WB 50 µl
Rabbit anti PI-3 kinase p85 subunit alpha antibody recognizes PI-3 kinase p85 subunit alpha. PI-3 kinase p85 subunit alpha is a regulatory subunit of PI 3-kinase family of enzymes. PI 3-kinases are involved in a variety of cellular functions as part of the PI3K/AKT/mTOR pathway.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to PI-3 kinase p85 subunit alpha was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant human PI-3 kinase p85 subunit alpha

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P27986 Related reagents
Entrez Gene
PIK3R1 Related reagents
GO Terms
GO:0043125 ErbB-3 class receptor binding
GO:0005158 insulin receptor binding
GO:0005159 insulin-like growth factor receptor binding
GO:0005545 1-phosphatidylinositol binding
GO:0005943 1-phosphatidylinositol-4-phosphate 3-kinase, class IA complex
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0014065 phosphatidylinositol 3-kinase cascade
GO:0046326 positive regulation of glucose import
GO:0019903 protein phosphatase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0035014 phosphatidylinositol 3-kinase regulator activity
GO:0043559 insulin binding
GO:0043560 insulin receptor substrate binding
GO:0044419 interspecies interaction between organisms
GO:0046854 phosphatidylinositol phosphorylation
GO:0048009 insulin-like growth factor receptor signaling pathway
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0050900 leukocyte migration
GO:0060396 growth hormone receptor signaling pathway
GO:0090004 positive regulation of establishment of protein localization in plasma membrane
Regulatory
For research purposes only

Applications of PI-3 Kinase p85 Subunit Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti PI-3 kinase p85 subunit alpha antibody detects a band of approximately 85 kDa in THP-1 cell lysate under reducing conditions

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*