PI-3 Kinase p110 Subunit Alpha antibody

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Goat anti Human PI-3 Kinase p110 Subunit Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1898 E P 0.1 mg
Goat anti Human PI-3 kinase p110 subunit alpha antibody
PI3-kinase is a heterodimer consisting of a p85 regulatory subunit and a p110 catalytic subunit, which itself is divided into isoforms p110alpha, p110beta, p110delta and p110gamma. Mutations in the PIK3CA gene are associated with breast, ovarian and colorectal cancers (CRC).http://www.omim.org/entry/114500 Colorectal cancer is a heterogeneous disease that is common in both men and women. characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum (Vehlo et al. 2005).https://www.ncbi.nlm.nih.gov/pubmed/15994075

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
Dog Expected from Sequence
Bovine Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human p110alpha was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide C-HQWLKDKNKGEIYD from the internal region of human PI3-kinase p110 subunit alpha (NP_006209.2).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P42336 Related reagents
Entrez Gene
PIK3CA Related reagents
GO Terms
GO:0005524 ATP binding
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0016303 1-phosphatidylinositol-3-kinase activity
GO:0046934 phosphatidylinositol-4,5-bisphosphate 3-kinase activity
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0050900 leukocyte migration
Regulatory
For research purposes only

Applications of PI-3 Kinase p110 Subunit Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/128000
Immunohistology - Paraffin 2.0 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human cerebral cortex

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P