PI-3 Kinase p110 Subunit Alpha antibody

Goat anti Human PI-3 Kinase p110 Subunit Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
PI-3 Kinase p110 Subunit Alpha

Product Code Applications Pack Size List Price Your Price Qty
AHP1898
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P 0.1 mg
List Price Your Price
Search for Batch Specific Datasheets

Goat anti Human PI-3 kinase p110 subunit alpha antibody recognizes human PI3-kinase p110 subunit alpha (Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha), otherwise known as PIK3CA, a member of the PI3/PI4-kinase family of lipid kinases, implicated in the regulation of signal transduction during cell growth.

PI3-kinase is a heterodimer consisting of a p85 regulatory subunit and a p110 catalytic subunit, which itself is divided into isoforms p110alpha, p110beta, p110delta and p110gamma. Mutations in the PIK3CA gene are associated with breast, ovarian and colorectal cancers (CRC).http://www.omim.org/entry/114500 Colorectal cancer is a heterogeneous disease that is common in both men and women. characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum (Vehlo et al. 2005).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
Dog Expected from Sequence
Bovine Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human p110alpha was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Synthetic peptide C-HQWLKDKNKGEIYD from the internal region of human PI3-kinase p110 subunit alpha (NP_006209.2).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/128000
Immunohistology - Paraffin 2.0 3.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human cerebral cortex

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Description Rabbit anti Goat IgG (Fc):HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
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Description Antigen Retrieval Buffer, pH8.0

Synonyms
PIK3CA
RRID
AB_2299726
UniProt
P42336
Entrez Gene
PIK3CA
GO Terms
GO:0005524 ATP binding
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0008286 insulin receptor signaling pathway
GO:0008543 fibroblast growth factor receptor signaling pathway
GO:0016303 1-phosphatidylinositol-3-kinase activity
GO:0046934 phosphatidylinositol-4,5-bisphosphate 3-kinase activity
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0050900 leukocyte migration
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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