Phospholamban (Cardiac) antibody

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Rabbit anti Phospholamban (Cardiac)

Rabbit anti human phospholamban (cardiac) antibody recognizes the protein encoded by the PLN gene found in cardiac muscle. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00355 WB 100 µl
Rabbit anti Human phospholamban (cardiac) antibody recognizes phospholamban (cardiac).

Phospholamban (cardiac) is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. It is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein itself. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. Phospholamban (cardiac) is a key regulator of cardiac diastolic function. Mutations in Phospholamban (cardiac) gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure (provided by RefSeq, Jul 2008).

Rabbit anti Human phospholamban (cardiac) antibody detects bands of 12 and 24 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti phospholamban (cardiac) detects bands of approximately 12 and 24 kDa in mouse heart tissue
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 1-22 of human phospholamban (cardiac)
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P26678 Related reagents
Entrez Gene
PLN Related reagents
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0005246 calcium channel regulator activity
GO:0008015 blood circulation
GO:0016529 sarcoplasmic reticulum
GO:0031966 mitochondrial membrane
GO:0051924 regulation of calcium ion transport
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Phospholamban (Cardiac) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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