PHGDH antibody

Rabbit anti PHGDH

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
PHGDH

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VPA00398
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Rabbit anti Human PHGDH antibody recognizes PHGDH, also known as 3-phosphoglycerate dehydrogenase, NLS1, epididymis secretory protein Li 113 and HEL-S-113.

The PHGDH gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in PHGDH have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known (provided by RefSeq, Aug 2011).

Rabbit anti Human PHGDH antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti PHGDH detects a band of approximately 57 kDa in NIH/3T3 cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 249-277 of human PHGDH
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
O43175
Entrez Gene
PHGDH
GO Terms
GO:0009055 electron carrier activity
GO:0004617 phosphoglycerate dehydrogenase activity
GO:0055114 oxidation-reduction process
GO:0006564 L-serine biosynthetic process
GO:0007420 brain development
GO:0051287 NAD binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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