PHAP I antibody

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Rabbit anti PHAP I

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00089
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human PHAP I antibody recognizes putative HLA-DR-associated protein I (PHAP I), also known as acidic leucine-rich nuclear phosphoprotein 32 family member A.

PHAP I is a 249 amino acid member of the ANP32 family, containing 4 leucine rich repeats and a single LRRCT domain. This protein plays a role in a number of cellular processes, including proliferation, differentiation, apoptosis and tumor suppression. PHAP I is involved in the activation of caspase-9 following apatosome formation (Chiang et al. 2003). PHAP I is ubiquitously expressed with principally nuclear expression.

Rabbit anti Human PHAP I antibody detects putative HLA-DR-associated protein I as a single band of 29 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Rabbit anti PHAP I detects a band of approximately 29 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to amino acids at the carboxy terminus of human PHAP I.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P39687
Entrez Gene
ANP32A
GO Terms
GO:0005515 protein binding
GO:0005783 endoplasmic reticulum
GO:0035556 intracellular signal transduction
GO:0005654 nucleoplasm
GO:0006350 transcription
GO:0006913 nucleocytoplasmic transport
GO:0016071 mRNA metabolic process
GO:0045449 regulation of transcription
GO:0048471 perinuclear region of cytoplasm
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PHAP I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml