PHAP I antibody
Rabbit anti PHAP I
PHAP I antibody recognizes the acidic leucine-rich nuclear phosphoprotein 32 family member A, also known as acidic nuclear phosphoprotein pp32, pp32, inhibitor I1PP2A Putative HLA-DR-associated protein I or PHAPI. The antibody has been validated for western blotting using whole cell lysates.
PHAP I Antibody with Control Lysate
PHAP I antibody recognizes the Acidic leucine-rich nuclear phosphoprotein 32 family member A, also known as acidic nuclear phosphoprotein pp32, pp32, leucine-rich acidic nuclear protein, LANP, mapmodulin potent heat-stable protein phosphatase 2A, or PHAPI.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00089 | WB | 100 µl | ||||
| VPA00089KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human PHAP I antibody recognizes putative HLA-DR-associated protein I (PHAP I), also known as acidic leucine-rich nuclear phosphoprotein 32 family member A.
PHAP I is a 249 amino acid member of the ANP32 family, containing 4 leucine rich repeats and a single LRRCT domain. This protein plays a role in a number of cellular processes, including proliferation, differentiation, apoptosis and tumor suppression. PHAP I is involved in the activation of caspase-9 following apatosome formation (Chiang et al. 2003). PHAP I is ubiquitously expressed with principally nuclear expression.
Rabbit anti Human PHAP I antibody detects putative HLA-DR-associated protein I as a single band of 29 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
PHAP I is a 249 amino acid member of the ANP32 family, containing 4 leucine rich repeats and a single LRRCT domain. This protein plays a role in a number of cellular processes, including proliferation, differentiation, apoptosis and tumor suppression. PHAP I is involved in the activation of caspase-9 following apatosome formation (Chiang et al. 2003). PHAP I is ubiquitously expressed with principally nuclear expression.
Rabbit anti Human PHAP I antibody detects putative HLA-DR-associated protein I as a single band of 29 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti PHAP I detects a band of approximately 29 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- A peptide corresponding to amino acids at the carboxy terminus of human PHAP I.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P39687 Related reagents
- Entrez Gene
- ANP32A Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0005783 endoplasmic reticulum
- GO:0035556 intracellular signal transduction
- GO:0005654 nucleoplasm
- GO:0006350 transcription
- GO:0006913 nucleocytoplasmic transport
- GO:0016071 mRNA metabolic process
- GO:0045449 regulation of transcription
- GO:0048471 perinuclear region of cytoplasm
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of PHAP I antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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