PHAP I antibody

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Rabbit anti PHAP I

PHAP I antibody recognizes the acidic leucine-rich nuclear phosphoprotein 32 family member A, also known as acidic nuclear phosphoprotein pp32, pp32, inhibitor I1PP2A Putative HLA-DR-associated protein I or PHAPI. The antibody has been validated for western blotting using whole cell lysates.

PHAP I Antibody with Control Lysate

PHAP I antibody recognizes the Acidic leucine-rich nuclear phosphoprotein 32 family member A, also known as acidic nuclear phosphoprotein pp32, pp32, leucine-rich acidic nuclear protein, LANP, mapmodulin potent heat-stable protein phosphatase 2A, or PHAPI.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00089 WB 100 µl
VPA00089KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human PHAP I antibody recognizes putative HLA-DR-associated protein I (PHAP I), also known as acidic leucine-rich nuclear phosphoprotein 32 family member A.

PHAP I is a 249 amino acid member of the ANP32 family, containing 4 leucine rich repeats and a single LRRCT domain. This protein plays a role in a number of cellular processes, including proliferation, differentiation, apoptosis and tumor suppression. PHAP I is involved in the activation of caspase-9 following apatosome formation (Chiang et al. 2003). PHAP I is ubiquitously expressed with principally nuclear expression.

Rabbit anti Human PHAP I antibody detects putative HLA-DR-associated protein I as a single band of 29 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Rabbit anti PHAP I detects a band of approximately 29 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to amino acids at the carboxy terminus of human PHAP I.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P39687 Related reagents
Entrez Gene
ANP32A Related reagents
GO Terms
GO:0005515 protein binding
GO:0005783 endoplasmic reticulum
GO:0035556 intracellular signal transduction
GO:0005654 nucleoplasm
GO:0006350 transcription
GO:0006913 nucleocytoplasmic transport
GO:0016071 mRNA metabolic process
GO:0045449 regulation of transcription
GO:0048471 perinuclear region of cytoplasm
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PHAP I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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