PHAP I antibody

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Rabbit anti PHAP I (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1664
Datasheet
P* WB
MSDS
0.1 mg loader

Rabbit anti PHAP I (C-Terminal) antibody recognizes the carboxy Terminal of human Acidic leucine-rich nuclear phosphoprotein 32 family member A, also known as putative LHA-DR-associated protein, PHAP I, Acidic nuclear phosphoprotein pp32, Leucine-rich acidic nuclear protein, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A or Mapmodulin. PHAP I is a249 amino acid member of the ANP32 family , containing 4 leucine rich repeats and a single LRRCT domain.. PHAP I plays a role in a number of cellular processes, including proliferation, differentiation, apoptosis and tumour suppression. PHAp- 1 is involved in the activation of caspase-9 following apatosome formation (Chiang et al. 2003). PHAP 1 is ubiquitously expressed with principally nuclear expression.

Rabbit anti PHAP I (C-Terminal) antibody does not cross-react with other isoforms of PHAP.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to PHAP I (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by immunoaffinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to amino acids at the carboxy terminus of human PHAP I.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P39687
Entrez Gene
ANP32A
GO Terms
GO:0005515 protein binding
GO:0005783 endoplasmic reticulum
GO:0035556 intracellular signal transduction
GO:0005654 nucleoplasm
GO:0006350 transcription
GO:0006913 nucleocytoplasmic transport
GO:0016071 mRNA metabolic process
GO:0045449 regulation of transcription
GO:0048471 perinuclear region of cytoplasm
Regulatory
For research purposes only

Applications of PHAP I antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 2ug/ml
Western Blotting 0.5 2.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1664 detects a band of approximately 32kDa in Raji cell lysate

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml