PGK1 antibody

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Rabbit anti PGK1

Rabbit anti human PGK1 antibody recognizes phosphoglycerate kinase 1, also known as PRP2, cell migration-inducing gene 10 protein, epididymis secretory sperm binding protein Li 68p or primer recognition protein 2. This antibody has been validated for western blotting using whole cell lysates.

PGK1 Antibody with Control Lysate

Rabbit anti human PGK1 antibody recognizes phosphoglycerate kinase 1, also known as PRP2, cell migration-inducing gene 10 protein, epididymis secretory sperm binding protein Li 68p or primer recognition protein 2. This antibody has been extensively validated for western blotting.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00354 WB 100 µl
VPA00354KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human PGK1 antibody recognizes PGK1, also known as phosphoglycerate kinase 1, PRP 2, cell migration-inducing gene 10 protein, epididymis secretory sperm binding protein Li 68p and primer recognition protein 2.

The protein encoded by the PGK1 gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. PGK1 lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19 (provided by RefSeq, Oct 2008).

Rabbit anti Human PGK1 antibody detects a band of 45 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - liquid
Product Form
Ig fraction - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Preparation
20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 117-145 of human PGK1

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P00558 Related reagents
Entrez Gene
PGK1 Related reagents
GO Terms
GO:0004618 phosphoglycerate kinase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0006094 gluconeogenesis
GO:0006096 glycolysis
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PGK1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti PGK1 detects a band of approximately 45 kDa in NIH/3T3 cell lysate
Western Blotting
Anti PGK1 detects a band of approximately 45 kDa in NIH/3T3 cell lysate
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB