PGK1 antibody
Rabbit anti PGK1
Rabbit anti human PGK1 antibody recognizes phosphoglycerate kinase 1, also known as PRP2, cell migration-inducing gene 10 protein, epididymis secretory sperm binding protein Li 68p or primer recognition protein 2. This antibody has been validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00354 | WB | 100 µl |
Rabbit anti Human PGK1 antibody recognizes PGK1, also known as phosphoglycerate kinase 1, PRP 2, cell migration-inducing gene 10 protein, epididymis secretory sperm binding protein Li 68p and primer recognition protein 2.
The protein encoded by the PGK1 gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. PGK1 lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19 (provided by RefSeq, Oct 2008).
Rabbit anti Human PGK1 antibody detects a band of 45 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The protein encoded by the PGK1 gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. PGK1 lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19 (provided by RefSeq, Oct 2008).
Rabbit anti Human PGK1 antibody detects a band of 45 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti PGK1 detects a band of approximately 45 kDa in NIH/3T3 cell lysate
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Ig fraction - liquid
- Preparation
- Rabbit Ig fraction prepared by ammonium sulphate precipitation
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH-conjugated synthetic peptide corresponding to aa 117-145 of human PGK1
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Guarantee
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P00558 Related reagents
- Entrez Gene
- PGK1 Related reagents
- GO Terms
- GO:0004618 phosphoglycerate kinase activity
- GO:0005829 cytosol
- GO:0005524 ATP binding
- GO:0006094 gluconeogenesis
- GO:0006096 glycolysis
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of PGK1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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