PGK1 antibody

Rabbit anti PGK1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
PGK1

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VPA00354
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Rabbit anti Human PGK1 antibody recognizes PGK1, also known as phosphoglycerate kinase 1, PRP 2, cell migration-inducing gene 10 protein, epididymis secretory sperm binding protein Li 68p and primer recognition protein 2.

The protein encoded by the PGK1 gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. PGK1 lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19 (provided by RefSeq, Oct 2008).

Rabbit anti Human PGK1 antibody detects a band of 45 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti PGK1 detects a band of approximately 45 kDa in NIH/3T3 cell lysate.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 117-145 of human PGK1
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P00558
Entrez Gene
PGK1
GO Terms
GO:0004618 phosphoglycerate kinase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0006094 gluconeogenesis
GO:0006096 glycolysis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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