PGAM1 antibody

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Goat anti PGAM1

PGAM1 antibody recognizes phosphoglycerate mutase 1, also known as BPG-dependent PGAM 1, phosphoglycerate mutase isozyme B or PGAM-B. The antibody has been extensively validated for western blotting using whole cell lysates.

PGAM1 Antibody with Control Lysate

PGAM1 antibody recognizes phosphoglycerate mutase 1, also known as BPG-dependent PGAM 1, phosphoglycerate mutase isozyme B or PGAM-B. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00128 WB 100 µl
VPA00128KT WB 2 Westerns
With Lysate 400µg
Goat anti Human PGAM1 antibody recognizes phosphoglycerate mutase 1 (PGAM1), also known as BPG-dependent PGAM 1, phosphoglycerate mutase isozyme B or PGAM-B.

Phosphoglycerate mutase 1 is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway (summary by Chen et al., 1974 (PubMed 4811757)) (supplied by OMIM, Nov 2010).

Goat anti Human PGAM1 antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PGAM1 detects a band of approximately 28 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence C-KAMEAVAAQGKAKK, from the C Terminus of the protein sequence.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P18669 Related reagents
Entrez Gene
PGAM1 Related reagents
GO Terms
GO:0004082 bisphosphoglycerate mutase activity
GO:0004083 2,3-bisphospho-D-glycerate 2-phosphohydrolase activity
GO:0004619 phosphoglycerate mutase activity
GO:0005829 cytosol
GO:0006094 gluconeogenesis
GO:0006096 glycolysis
GO:0006110 regulation of glycolysis
GO:0019901 protein kinase binding
GO:0043456 regulation of pentose-phosphate shunt
GO:0045730 respiratory burst
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PGAM1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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