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PGAM1 antibody

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Western Blot Validated
Anti PGAM1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with PGAM1 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 6.261 second exposure. Arrow points to PGAM1 (molecular weight 28 kDa)

Goat anti PGAM1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
PrecisionAb Antibodies
Product Code Applications Pack Size List Price Quantity
VPA00128
Datasheet
WB
MSDS
100 µl

Goat anti Human PGAM1 antibody recognizes phosphoglycerate mutase 1 (PGAM1), also known as BPG-dependent PGAM 1, phosphoglycerate mutase isozyme B or PGAM-B.

Phosphoglycerate mutase 1 is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway (summary by Chen et al., 1974 (PubMed 4811757)) (supplied by OMIM, Nov 2010).

Goat anti Human PGAM1 antibody detects a band of 28 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PGAM1 detects a band of approximately 28 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence C-KAMEAVAAQGKAKK, from the C Terminus of the protein sequence.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P18669
Entrez Gene
PGAM1
GO Terms
GO:0004082 bisphosphoglycerate mutase activity
GO:0004083 2,3-bisphospho-D-glycerate 2-phosphohydrolase activity
GO:0004619 phosphoglycerate mutase activity
GO:0005829 cytosol
GO:0006094 gluconeogenesis
GO:0006096 glycolysis
GO:0006110 regulation of glycolysis
GO:0019901 protein kinase binding
GO:0043456 regulation of pentose-phosphate shunt
GO:0045730 respiratory burst
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PGAM1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

A Precise Solution to Reproducible Western Blots - PrecisionAb Antibodies
Request a different product with the PGAM1 specificity