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Peroxiredoxin 3 antibody

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Anti Peroxiredoxin 3 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1
Anti Peroxiredoxin 3 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with peroxiredoxin 3 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to peroxiredoxin 3 (molecular weight 23 kDa)

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Rabbit anti Peroxiredoxin 3

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
Peroxiredoxin 3

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Rabbit anti Human peroxiredoxin 3 antibody recognizes peroxiredoxin 3 also known as antioxidant protein 1 and protein MER5 homolog.

Peroxiredoxin 3 is a protein with antioxidant function and is localized in the mitochondrion. The PRDX3 gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of PRDX3 product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for PRDX3 (provided by RefSeq, Jul 2008).

Rabbit anti Human peroxiredoxin 3 antibody detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti peroxiredoxin 3 detects a band of approximately 23 kDa in HEK293 cell lysate.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 229-262 of human peroxiredoxin 3
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml
List Price Your Price
Description Goat anti Rabbit IgG (H/L):HRP

UniProt
P30048
Entrez Gene
PRDX3
GO Terms
GO:0005739 mitochondrion
GO:0004601 peroxidase activity
GO:0005769 early endosome
GO:0055114 oxidation-reduction process
GO:0045454 cell redox homeostasis
GO:0007005 mitochondrion organization
GO:0008022 protein C-terminus binding
GO:0008284 positive regulation of cell proliferation
GO:0008785 alkyl hydroperoxide reductase activity
GO:0019901 protein kinase binding
GO:0030099 myeloid cell differentiation
GO:0032496 response to lipopolysaccharide
GO:0033673 negative regulation of kinase activity
GO:0042744 hydrogen peroxide catabolic process
GO:0043027 caspase inhibitor activity
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051881 regulation of mitochondrial membrane potential
GO:0051920 peroxiredoxin activity
View more products with PEROXIREDOXIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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