Peroxiredoxin 3 antibody

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Anti Peroxiredoxin 3 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with peroxiredoxin 3 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to peroxiredoxin 3 (molecular weight 23 kDa)

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Rabbit anti Peroxiredoxin 3

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Peroxiredoxin 3

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VPA00389 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Rabbit anti Human peroxiredoxin 3 antibody recognizes peroxiredoxin 3 also known as antioxidant protein 1 and protein MER5 homolog.

Peroxiredoxin 3 is a protein with antioxidant function and is localized in the mitochondrion. The PRDX3 gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of PRDX3 product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for PRDX3 (provided by RefSeq, Jul 2008).

Rabbit anti Human peroxiredoxin 3 antibody detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti peroxiredoxin 3 detects a band of approximately 23 kDa in HEK293 cell lysate.
Product Form: Purified IgG - liquid
Preparation: Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: KLH-conjugated synthetic peptide corresponding to aa 229-262 of human peroxiredoxin 3
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Rabbit IgG (H/L):HRP	STAR208P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Rabbit IgG (H/L):HRP

UniProt: P30048
Entrez Gene: PRDX3
GO Terms: GO:0005739 mitochondrion; GO:0004601 peroxidase activity; GO:0005769 early endosome; GO:0055114 oxidation-reduction process; GO:0045454 cell redox homeostasis; GO:0007005 mitochondrion organization; GO:0008022 protein C-terminus binding; GO:0008284 positive regulation of cell proliferation; GO:0008785 alkyl hydroperoxide reductase activity; View More GO Terms; GO:0019901 protein kinase binding; GO:0030099 myeloid cell differentiation; GO:0032496 response to lipopolysaccharide; GO:0033673 negative regulation of kinase activity; GO:0042744 hydrogen peroxide catabolic process; GO:0043027 caspase inhibitor activity; GO:0051092 positive regulation of NF-kappaB transcription factor activity; GO:0051881 regulation of mitochondrial membrane potential; GO:0051920 peroxiredoxin activity