Peroxiredoxin 3 antibody

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Rabbit anti Peroxiredoxin 3

Rabbit anti human peroxiredoxin 3 antibody recognizes thioredoxin-dependent peroxide reductase, mitochondrial, also known as antioxidant protein 1, AOP1, peroxiredoxin III, PRDX3, peroxiredoxin-3 or protein MER5 homolog. This antibody has been validated for western blotting using whole cell lysates.

Peroxiredoxin 3 Antibody with Control Lysate

Rabbit anti human peroxiredoxin 3 antibody recognizes thioredoxin-dependent peroxide reductase, mitochondrial, also known as antioxidant protein 1, AOP1, peroxiredoxin III, PRDX3, peroxiredoxin-3 or protein MER5 homolog. This antibody has been extensively validated for western blotting.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00389 WB 100 µl
VPA00389KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human peroxiredoxin 3 antibody recognizes peroxiredoxin 3 also known as antioxidant protein 1 and protein MER5 homolog.

Peroxiredoxin 3 is a protein with antioxidant function and is localized in the mitochondrion. The PRDX3 gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of PRDX3 product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for PRDX3 (provided by RefSeq, Jul 2008).

Rabbit anti Human peroxiredoxin 3 antibody detects a band of 23 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti peroxiredoxin 3 detects a band of approximately 23 kDa in HEK293 cell lysate
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography
Preparation
20μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 229-262 of human peroxiredoxin 3
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P30048 Related reagents
Entrez Gene
PRDX3 Related reagents
GO Terms
GO:0005739 mitochondrion
GO:0004601 peroxidase activity
GO:0005769 early endosome
GO:0055114 oxidation-reduction process
GO:0045454 cell redox homeostasis
GO:0007005 mitochondrion organization
GO:0008022 protein C-terminus binding
GO:0008284 positive regulation of cell proliferation
GO:0008785 alkyl hydroperoxide reductase activity
GO:0019901 protein kinase binding
GO:0030099 myeloid cell differentiation
GO:0032496 response to lipopolysaccharide
GO:0033673 negative regulation of kinase activity
GO:0042744 hydrogen peroxide catabolic process
GO:0043027 caspase inhibitor activity
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051881 regulation of mitochondrial membrane potential
GO:0051920 peroxiredoxin activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Peroxiredoxin 3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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