Peroxiredoxin 2 antibody

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Rabbit anti Peroxiredoxin 2

Rabbit anti human peroxiredoxin 2 antibody recognizes the peroxiredoxin-2 protein, also known as epididymis secretory sperm binding protein Li 2a, natural killer cell-enhancing factor B, or torin. This antibody has been extensively validated for western blotting using whole cell lysates.

Peroxiredoxin 2 Antibody with Control Lysate

Rabbit anti human peroxiredoxin 2 antibody recognizes the peroxiredoxin-2 protein, also known as epididymis secretory sperm binding protein Li 2a, natural killer cell-enhancing factor B, thiol-specific antioxidant 1, thioredoxin peroxidase 1 or torin.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00330 WB 100 µl
VPA00330KT WB 2 Westerns
With Lysate 400µg
Rabbit anti Human peroxiredoxin 2 antibody recognizes peroxiredoxin 2, also known as epididymis secretory sperm binding protein Li 2a, natural killer cell-enhancing factor B, thiol-specific antioxidant 1, thioredoxin peroxidase 1, thioredoxin-dependent peroxide reductase 1 and torin.

Peroxiredoxin 2 is member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. This protein plays an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8(+) T-cells. The crystal structure of the protein has been resolved to 2.7 angstroms. This protein prevents hemolytic anemia from oxidative stress by stabilizing hemoglobin, thus making Peroxiredoxin 2 a therapeutic target for patients with hemolytic anemia. This protein may have a proliferative effect and play a role in cancer development or progression. Related pseudogenes have been identified on chromosomes 5, 6, 10 and 13 (provided by RefSeq, Mar 2013).

Rabbit anti Human peroxiredoxin 2 antibody detects a band of 22 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti peroxiredoxin 2 detects a band of approximately 22 kDa in LNCaP cell lysate
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - liquid
Product Form
Ig fraction - liquid
Preparation
Rabbit Ig fraction prepared by ammonium sulphate precipitation
Preparation
20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
KLH-conjugated synthetic peptide corresponding to aa 169-198 of human peroxiredoxin 2
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg LNCaP lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P32119 Related reagents
Entrez Gene
PRDX2 Related reagents
GO Terms
GO:0055114 oxidation-reduction process
GO:0045454 cell redox homeostasis
GO:0006916 anti-apoptosis
GO:0008379 thioredoxin peroxidase activity
GO:0019430 removal of superoxide radicals
GO:0042744 hydrogen peroxide catabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Peroxiredoxin 2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB
Goat anti Rabbit IgG (H/L):HRP STAR208P 2 ml WB

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