Peroxiredoxin 1 antibody

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Goat anti Peroxiredoxin 1 Antibody

Peroxiredoxin 1 antibody recognizes PRDX1, also known as natural killer cell-enhancing factor A, NKEF-A, proliferation-associated gene protein, PAG thioredoxin peroxidase 2 or thioredoxin-dependent peroxide reductase 2. The antibody has been validated for western blotting using whole cell lysates.

Peroxiredoxin 1 Antibody with Control Lysate

PRDX1 antibody recognizes Peroxiredoxin-1, also known as natural killer cell-enhancing factor A, NKEF-A, Proliferation-associated gene protein, PAG Thioredoxin peroxidase 2 or Thioredoxin-dependent peroxide reductase 2. The antibody has been extensively validated for western blotting.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00056 WB 100 µl
VPA00056KT WB 2 Westerns
With Lysate 400µg
Goat anti Human peroxiredoxin 1 antibody recognizes PRDX1 a 22 kDa protein and a member of the peroxiredoxin family of antioxidant enzymes. Members of the family are divided into the 1-Cys and 2-Cys groups based on the number of conserved residues used in the redox reaction. Peroxiredoxins reduce hydrogen peroxides and alkyl hydroperoxides and thus exert an anti-oxidant effect.

Goat anti Human peroxiredoxin 1 antibody detects peroxiredoxin 1 as a single band of 22 kDa in a wide range of human cell line lysates tested. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Goat anti peroxiredoxin 1 detects a band of approximately 22 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide with sequence C-SDPKRTIAQDYG from the internal region of the protein sequence.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q06830 Related reagents
Entrez Gene
PRDX1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0001501 skeletal system development
GO:0005739 mitochondrion
GO:0005634 nucleus
GO:0055114 oxidation-reduction process
GO:0045454 cell redox homeostasis
GO:0008283 cell proliferation
GO:0008379 thioredoxin peroxidase activity
GO:0042470 melanosome
GO:0042744 hydrogen peroxide catabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Peroxiredoxin 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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