Rabbit anti PDPK1 antibody recognizes 3-phosphoinositide dependent protein kinase-1, also known as PDPK1. This protein is implicated in the development and progression of melanomas. PDPK1 is a master kinase, which is crucial for the activation of AKT/PKB and many other AGC kinases including PKC, S6K, SGK.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 30% Glycerol
0.5% Bovine Serum Albumin
- Synthetic peptide surrounding amino acid 542 of human PDPK1
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
actin cytoskeleton organization
3-phosphoinositide-dependent protein kinase activity
negative regulation of protein kinase activity
insulin receptor signaling pathway
T cell costimulation
activation of protein kinase B activity
nerve growth factor receptor signaling pathway
T cell receptor signaling pathway
positive regulation of establishment of protein localization in plasma membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of PDPK1 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti PDPK1 antibody recognizes an approximately 60kDa as well as a 33kDa band, representing the cleavage product in Jurkat lysates
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Secondary Antibodies Available
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