PDPK1 antibody

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Rabbit anti PDPK1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2337
Datasheet
IP P WB
MSDS
50 µg loader

Rabbit anti PDPK1 antibody recognizes 3-phosphoinositide dependent protein kinase-1, also known as PDPK1. This protein is implicated in the development and progression of melanomas. PDPK1 is a master kinase, which is crucial for the activation of AKT/PKB and many other AGC kinases including PKC, S6K, SGK.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
30% Glycerol
0.5% Bovine Serum Albumin
0.01% Thiomersal
Immunogen
Synthetic peptide surrounding amino acid 542 of human PDPK1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O15530
Entrez Gene
PDPK1
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0030036 actin cytoskeleton organization
GO:0005829 cytosol
GO:0004676 3-phosphoinositide-dependent protein kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006469 negative regulation of protein kinase activity
GO:0007268 synaptic transmission
GO:0008286 insulin receptor signaling pathway
GO:0018107 peptidyl-threonine phosphorylation
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0032148 activation of protein kinase B activity
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0090004 positive regulation of establishment of protein localization in plasma membrane
Regulatory
For research purposes only

Applications of PDPK1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 15 25 ug/ml
Immunoprecipitation 10 20 ug/ml
Western Blotting 0.5 4.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti PDPK1 antibody recognizes an approximately 60kDa as well as a 33kDa band, representing the cleavage product in Jurkat lysates

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg