PDK1 (pSer241) antibody

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Rabbit anti PDK1 (pSer241)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2669
Datasheet
P WB
MSDS
50 µl loader

Rabbit anti PDK1 (pSer241) antibody recognizes PDK1, also known as 3-phosphoinositide-dependent protein kinase 1 or PDPK1, when phosphorylated at serine 241. PDK1 is a master kinase involved in numerous cell processes including proliferation, differentiation and cell survival. PDK1 is crucial for the activation of AKT/PKB and many other AGC kinases including PKC, S6K, SGK.

Autophosphorylation of serine 241 is required for PDK1 activity (Wang et al. 2017).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to PDK1 (pSer241) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 241 of human PDK1

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O15530
Entrez Gene
PDPK1
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0030036 actin cytoskeleton organization
GO:0005829 cytosol
GO:0004676 3-phosphoinositide-dependent protein kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006469 negative regulation of protein kinase activity
GO:0007268 synaptic transmission
GO:0008286 insulin receptor signaling pathway
GO:0018107 peptidyl-threonine phosphorylation
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030168 platelet activation
GO:0031295 T cell costimulation
GO:0032148 activation of protein kinase B activity
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0090004 positive regulation of establishment of protein localization in plasma membrane
Regulatory
For research purposes only

Applications of PDK1 (pSer241) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/50 1/100
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Wang, X.Q. et al. (2017) CDK1-PDK1-PI3K/Akt signaling pathway regulates embryonic and induced pluripotency.
    Cell Death Differ. 24 (1): 38-48.