Rabbit anti PDK1 (pSer241) antibody
recognizes PDK1, also known as 3-phosphoinositide-dependent protein kinase 1 or PDPK1, when phosphorylated at serine 241. PDK1 is a master kinase involved in numerous cell processes including proliferation, differentiation and cell survival. PDK1 is crucial for the activation of AKT/PKB and many other AGC kinases including PKC, S6K, SGK.
Autophosphorylation of serine 241 is required for PDK1 activity (Wang et al. 2017
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
||Expected from Sequence
||Expected from Sequence
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to PDK1 (pSer241) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 241 of human PDK1
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
actin cytoskeleton organization
3-phosphoinositide-dependent protein kinase activity
negative regulation of protein kinase activity
insulin receptor signaling pathway
T cell costimulation
activation of protein kinase B activity
nerve growth factor receptor signaling pathway
T cell receptor signaling pathway
positive regulation of establishment of protein localization in plasma membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of PDK1 (pSer241) antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Wang, X.Q. et al. (2017) CDK1-PDK1-PI3K/Akt signaling pathway regulates embryonic and induced pluripotency.
Cell Death Differ. 24 (1): 38-48.