PDIA3 antibody

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Anti PDIA3 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with PDIA3 antibody followed by detection with Donkey anti Sheep/Goat IgG (Fc):HRP (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 119 second exposure. Arrow points to PDIA3 (molecular weight 58 kDa).

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Goat anti PDIA3

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: PDIA3

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Goat anti Human PDIA3 antibody recognizes protein disulfide-isomerase A3, also known as 58 kDa glucose-regulated protein, 58 kDa microsomal protein, disulfide isomerase ER-60, epididymis secretory protein Li 269 or phospholipase C-alpha.

The PDIA3 gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates (Trombetta and Helenius 1998) (provided by RefSeq, Jul 2008).

Goat anti Human PDIA3 antibody detects a band of 58 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti PDIA3 detects a band of approximately 58 kDa in HepG2 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin.
Immunogen: Peptide sequence C-TEYSALEQSTK
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Donkey anti Sheep/Goat IgG:HRP	STAR88P	C E P WB	1 ml	Log in	Get Quote
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Description	Donkey anti Sheep/Goat IgG:HRP

UniProt: P30101
Entrez Gene: PDIA3
GO Terms: GO:0005515 protein binding; GO:0004197 cysteine-type endopeptidase activity; GO:0006457 protein folding; GO:0003756 protein disulfide isomerase activity; GO:0006606 protein import into nucleus; GO:0004629 phospholipase C activity; GO:0007165 signal transduction; GO:0005788 endoplasmic reticulum lumen; GO:0045454 cell redox homeostasis; View More GO Terms; GO:0006621 protein retention in ER lumen; GO:0018279 protein N-linked glycosylation via asparagine; GO:0042470 melanosome; GO:0043687 post-translational protein modification