PDIA3 antibody

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Goat anti PDIA3

Goat anti-human PDIA3 antibody recognizes protein disulfide-isomerase A3, also known as 58 kDa glucose-regulated protein, 58 kDa microsomal protein, ER protein 57, ER protein 60, endoplasmic reticulum P58 or endoplasmic reticulum resident protein 57.

PDIA3 Antibody with Control Lysate

Goat anti-human PDIA3 antibody recognizes protein disulfide-isomerase A3, also known as 58 kDa glucose-regulated protein, 58 kDa microsomal protein, ER protein 57, ER protein 60, endoplasmic reticulum P58 or endoplasmic reticulum resident protein 57.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00513 WB 100 µl
VPA00513KT WB 2 Westerns
With Lysate 400µg
Goat anti Human PDIA3 antibody recognizes protein disulfide-isomerase A3, also known as 58 kDa glucose-regulated protein, 58 kDa microsomal protein, disulfide isomerase ER-60, epididymis secretory protein Li 269 or phospholipase C-alpha.

The PDIA3 gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates (Trombetta and Helenius 1998) (provided by RefSeq, Jul 2008).

Goat anti Human PDIA3 antibody detects a band of 58 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti PDIA3 detects a band of approximately 58 kDa in HepG2 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-TEYSALEQSTK
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HepG2 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P30101 Related reagents
Entrez Gene
PDIA3 Related reagents
GO Terms
GO:0005515 protein binding
GO:0004197 cysteine-type endopeptidase activity
GO:0006457 protein folding
GO:0003756 protein disulfide isomerase activity
GO:0006606 protein import into nucleus
GO:0004629 phospholipase C activity
GO:0007165 signal transduction
GO:0005788 endoplasmic reticulum lumen
GO:0045454 cell redox homeostasis
GO:0006621 protein retention in ER lumen
GO:0018279 protein N-linked glycosylation via asparagine
GO:0042470 melanosome
GO:0043687 post-translational protein modification
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PDIA3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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