PDGF antibody
Rabbit anti Human PDGF BB Homodimer antibody recognizes human PDGF-BB (Platelet derived growth factor B chain), a disulphide-linked beta-chain homodimer and member of the PDGF family, released from platelet alpha-granules following trauma/platelet activation.
Naturally occurring active forms of PDGF exist as dimers of two alpha chains (AA), two beta chains (BB), or a combination of both chains (AB), all of which signal through the high-affinity PDGF-Ralpha receptor, and the latter two of which can also signal through the PDGF-Rbeta receptor. PDGFs are potent mitogens for a variety of cells including those of the connective tissue, bone and cartilage, blood and smooth muscle cells, and are involved in several biological processes including chemotaxis, hyperplasia and embryonic neurogenesis.
The expression of PDGF-BB is associated with autocrine growth stimulation and the formation of various tumour types, with several studies focusing on dermatofibrosarcoma protuberans (DP), an infiltrating skin tumour arising from a translocation occurring between chromosome 22 (the location of the PDGFB gene) and chromosome 17 (the location of the COL1A1 gene), which results in the generation of the COL1A1-PDGFB chimeric protein.
Naturally occurring active forms of PDGF exist as dimers of two alpha chains (AA), two beta chains (BB), or a combination of both chains (AB), all of which signal through the high-affinity PDGF-Ralpha receptor, and the latter two of which can also signal through the PDGF-Rbeta receptor. PDGFs are potent mitogens for a variety of cells including those of the connective tissue, bone and cartilage, blood and smooth muscle cells, and are involved in several biological processes including chemotaxis, hyperplasia and embryonic neurogenesis.
The expression of PDGF-BB is associated with autocrine growth stimulation and the formation of various tumour types, with several studies focusing on dermatofibrosarcoma protuberans (DP), an infiltrating skin tumour arising from a translocation occurring between chromosome 22 (the location of the PDGFB gene) and chromosome 17 (the location of the COL1A1 gene), which results in the generation of the COL1A1-PDGFB chimeric protein.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human PDGF-BB were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present.
- Immunogen
- Recombinant human PDGF-BB
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of reconstitution.
More Information
- UniProt
- P01127
- Entrez Gene
- PDGFB
- GO Terms
- GO:0000139 Golgi membrane
- GO:0001938 positive regulation of endothelial cell proliferation
- GO:0002548 monocyte chemotaxis
- GO:0002576 platelet degranulation
- GO:0005576 extracellular region
- GO:0005161 platelet-derived growth factor receptor binding
- GO:0005518 collagen binding
- GO:0005788 endoplasmic reticulum lumen
- GO:0008083 growth factor activity
- GO:0009986 cell surface
- GO:0010512 negative regulation of phosphatidylinositol biosynthetic process
- GO:0010544 negative regulation of platelet activation
- GO:0014911 positive regulation of smooth muscle cell migration
- GO:0030168 platelet activation
- GO:0031093 platelet alpha granule lumen
- GO:0042803 protein homodimerization activity
- GO:0043406 positive regulation of MAP kinase activity
- GO:0043498 cell surface binding
- GO:0043536 positive regulation of blood vessel endothelial cell migration
- GO:0045740 positive regulation of DNA replication
- GO:0046982 protein heterodimerization activity
- GO:0048008 platelet-derived growth factor receptor signaling pathway
- GO:0048146 positive regulation of fibroblast proliferation
- GO:0048407 platelet-derived growth factor binding
- GO:0050921 positive regulation of chemotaxis
- GO:0051781 positive regulation of cell division
- Regulatory
- For research purposes only
Applications of PDGF antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5 | 2.0ug/ml | |
| Functional Assays | 0.03 | 0.04ug/ml | |
| Immunohistology - Paraffin 1 | |||
| Western Blotting | 0.1 | 0.2ug/ml |
- 1 This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
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| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Rao, C.D. et al. (1986) Structure and sequence of the human c-sis/platelet-derived growth factor 2 (SIS/PDGF2) transcriptional unit.
Proc Natl Acad Sci U S A. 83 (8): 2392-6. -
Simon, M.P. et al. (2001) Structural and functional analysis of a chimeric protein COL1A1-PDGFB generated by the translocation t(17;22)(q22;q13.1) in Dermatofibrosarcoma protuberans (DP).
Oncogene. 20 (23): 2965-75.
