PDGF antibody
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P ResetRabbit anti Human PDGF AA Homodimer
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- PDGF
- Region
- AA HOMODIMER
Antibody Quality and Performance Guarantee
| Rabbit anti Human PDGF AA Homodimer antibody recognizes human PDGF-AA (Platelet derived growth factor A chain), a disulphide-linked alpha-chain homodimer and member of the PDGF family, released from platelet alpha-granules following trauma/platelet activation. Naturally occurring active forms of PDGF exist as dimers of two alpha chains (AA), two beta chains (BB), or a combination of both chains (AB), all of which signal through the high-affinity PDGF-Ralpha receptor, and the latter two of which can also signal through the PDGF-Rbeta receptor. PDGFs are potent mitogens for a variety of cells including those of the connective tissue, bone and cartilage, blood and smooth muscle cells, and are involved in several biological processes including chemotaxis, hyperplasia and embryonic neurogenesis. Increased expression of PDGF-AA has been associated with a diverse range of conditions including progression of osteosarcomas, persistent Cytomegalovirus infection in kidney allografts and mediation of IL-13-stimulated lung fibroblast proliferation in asthma. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB. For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human PDGF-AA were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human PDGF-AA.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5ug/ml | |
| Functional Assays | |
0.17 | 0.19ug/ml |
| Immunohistology - Paraffin 1 | |
||
| Western Blotting | |
0.1 | 0.2ug/ml |
- 1 This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
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Further Reading
-
Rorsman, F. et al. (1988) Structural characterization of the human platelet-derived growth factor A-chain cDNA and gene: alternative exon usage predicts two different precursor proteins.
Mol Cell Biol. 8 (2): 571-7. -
Ingram, J.L. et al. (2004) IL-13 and IL-1beta promote lung fibroblast growth through coordinated up-regulation of PDGF-AA and PDGF-Ralpha.
FASEB J. 18 (10): 1132-4.
- Synonyms
- Platelet Derived Growth Factor AA
- RRID
- AB_844648
- UniProt
- P04085
- Entrez Gene
- PDGFA
- GO Terms
- GO:0000139 Golgi membrane
- GO:0002576 platelet degranulation
- GO:0005161 platelet-derived growth factor receptor binding
- GO:0005518 collagen binding
- GO:0005615 extracellular space
- GO:0005788 endoplasmic reticulum lumen
- GO:0009790 embryo development
- GO:0008083 growth factor activity
- GO:0009986 cell surface
- View More GO Terms
- GO:0010512 negative regulation of phosphatidylinositol biosynthetic process
- GO:0010544 negative regulation of platelet activation
- GO:0014910 regulation of smooth muscle cell migration
- GO:0030168 platelet activation
- GO:0030335 positive regulation of cell migration
- GO:0031093 platelet alpha granule lumen
- GO:0032956 regulation of actin cytoskeleton organization
- GO:0042803 protein homodimerization activity
- GO:0043498 cell surface binding
- GO:0045740 positive regulation of DNA replication
- GO:0046982 protein heterodimerization activity
- GO:0048008 platelet-derived growth factor receptor signaling pathway
- GO:0048146 positive regulation of fibroblast proliferation
- GO:0048407 platelet-derived growth factor binding
- GO:0050919 negative chemotaxis
- GO:0051781 positive regulation of cell division
AHP792
154749
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