PDCD4 antibody
Rabbit anti PDCD4
PDCD4 antibody recognizes programmed cell death protein 4, also known as neoplastic transformation inhibitor protein, nuclear antigen H731 or protein 197/15a. This antibody has been extensively validated for western blotting using whole cell lysates.
PDCD4 Antibody with Control Lysate
PDCD4 antibody recognizes programmed cell death protein 4, also known as neoplastic transformation inhibitor protein, nuclear antigen H731 or protein 197/15a. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00093 | WB | 100 µl | ||||
| VPA00093KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human PDCD4 antibody recognizes programmed cell death 4 (PDCD4), a novel tumor suppressor.
PDCD4 interacts with the RNA helicase eIF4A and the scaffolding protein eIF4G, two components of the translation initiation complex, and thus inhibits protein synthesis (Yang et al. 2003). PDCD4 has also been shown to inhibit transcription factor activator protein-1 (AP-1), which regulates several events required for cell invasion, including expression of matrix metalloproteinases and cell motility (Wang et al. 2013).
PDCD4 localizes to the nucleus in proliferating cells, and its expression is modulated by cytokines in natural killer and T-cells. PDCD4 expression is down regulated with tumor progression in carcinomas of the lung, colon, prostate and breast.
Rabbit anti Human PDCD4 antibody detects programmed cell death 4 as a single band of 52 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
PDCD4 interacts with the RNA helicase eIF4A and the scaffolding protein eIF4G, two components of the translation initiation complex, and thus inhibits protein synthesis (Yang et al. 2003). PDCD4 has also been shown to inhibit transcription factor activator protein-1 (AP-1), which regulates several events required for cell invasion, including expression of matrix metalloproteinases and cell motility (Wang et al. 2013).
PDCD4 localizes to the nucleus in proliferating cells, and its expression is modulated by cytokines in natural killer and T-cells. PDCD4 expression is down regulated with tumor progression in carcinomas of the lung, colon, prostate and breast.
Rabbit anti Human PDCD4 antibody detects programmed cell death 4 as a single band of 52 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.
Product Details
- Target Species
- Human
- Western Blotting
- Rabbit anti PDCD4 detects a band of approximately 52 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- 100μl Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to amino acids near the C-terminal of human PDCD4
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q53EL6 Related reagents
- Entrez Gene
- PDCD4 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0003723 RNA binding
- GO:0006915 apoptosis
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0007569 cell aging
- GO:0016481 negative regulation of transcription
- GO:0043508 negative regulation of JUN kinase activity
- GO:0045786 negative regulation of cell cycle
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of PDCD4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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