PDCD4 antibody

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Rabbit anti PDCD4

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00093
Datasheet
WB
MSDS
100 µl loader

Rabbit anti Human PDCD4 antibody recognizes programmed cell death 4 (PDCD4), a novel tumor suppressor.

PDCD4 interacts with the RNA helicase eIF4A and the scaffolding protein eIF4G, two components of the translation initiation complex, and thus inhibits protein synthesis (Yang et al. 2003). PDCD4 has also been shown to inhibit transcription factor activator protein-1 (AP-1), which regulates several events required for cell invasion, including expression of matrix metalloproteinases and cell motility (Wang et al. 2013).

PDCD4 localizes to the nucleus in proliferating cells, and its expression is modulated by cytokines in natural killer and T-cells. PDCD4 expression is down regulated with tumor progression in carcinomas of the lung, colon, prostate and breast.

Rabbit anti Human PDCD4 antibody detects programmed cell death 4 as a single band of 52 kDa in a wide range of human cell line lysates. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Rabbit anti PDCD4 detects a band of approximately 52 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
100μl Rabbit polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids near the C-terminal of human PDCD4

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
Q53EL6
Entrez Gene
PDCD4
GO Terms
GO:0005515 protein binding
GO:0003723 RNA binding
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0005634 nucleus
GO:0007569 cell aging
GO:0016481 negative regulation of transcription
GO:0043508 negative regulation of JUN kinase activity
GO:0045786 negative regulation of cell cycle
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of PDCD4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml