Rabbit anti PCF11 antibody recognizes pre-mRNA cleavage complex 2 protein Pcf11, also known as KIAA0824.
PCF11 is important for 3' processing of pre-mRNA and transcription termination, links transcription with mRNA export, and can selectively attenuate expression of transcriptional regulators (Kamieniarz-Gdula et al. 2019). PCF11 is autoregulated, and removal of the regulatory mechanism leads to global activation of polyadenylation sites close to promotors (Wang et al. 2019).
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to PCF11 was raised by repeated immunization of rabbits with highly purified antigen.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Recombinant fusion protein containing a sequence corresponding to amino acids 1256-1555 of human PCF11 (NP_056969.2)
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
nuclear mRNA splicing, via spliceosome
mRNA cleavage factor complex
termination of RNA polymerase II transcription
mRNA 3'-end processing
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of PCF11 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available