PCF11 antibody

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Anti PCF11 Antibody gallery image 1 — Western blot analysis was performed by loading 25µg of various whole cell lysates probed with Rabbit anti PCF11 (**AHP3003**) at 1/1000 dilution. Arrow points to PCF11 (molecular weight 180 kDa).

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Rabbit anti PCF11

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: PCF11

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AHP3003 Datasheet Datasheet SDS SDS	WB	50 µl		Log in	Get Quote
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Rabbit anti PCF11 antibody recognizes pre-mRNA cleavage complex 2 protein Pcf11, also known as KIAA0824.

PCF11 is important for 3' processing of pre-mRNA and transcription termination, links transcription with mRNA export, and can selectively attenuate expression of transcriptional regulators (Kamieniarz-Gdula et al. 2019). PCF11 is autoregulated, and removal of the regulatory mechanism leads to global activation of polyadenylation sites close to promotors (Wang et al. 2019).

Target Species: Human
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to PCF11 was raised by repeated immunization of rabbits with highly purified antigen.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide
50% Glycerol
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1256-1555 of human PCF11 (NP_056969.2)
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/500	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt: O94913
Entrez Gene: PCF11
GO Terms: GO:0000398 nuclear mRNA splicing, via spliceosome; GO:0005849 mRNA cleavage factor complex; GO:0006369 termination of RNA polymerase II transcription; GO:0006379 mRNA cleavage; GO:0031124 mRNA 3'-end processing