PCF11 antibody

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Rabbit anti PCF11

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP3003
Datasheet
WB
MSDS
50 µl loader

Rabbit anti PCF11 antibody recognizes pre-mRNA cleavage complex 2 protein Pcf11, also known as KIAA0824.

PCF11 is important for 3' processing of pre-mRNA and transcription termination, links transcription with mRNA export, and can selectively attenuate expression of transcriptional regulators (Kamieniarz-Gdula et al. 2019). PCF11 is autoregulated, and removal of the regulatory mechanism leads to global activation of polyadenylation sites close to promotors (Wang et al. 2019).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to PCF11 was raised by repeated immunization of rabbits with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1256-1555 of human PCF11 (NP_056969.2)

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O94913
Entrez Gene
PCF11
GO Terms
GO:0000398 nuclear mRNA splicing, via spliceosome
GO:0005849 mRNA cleavage factor complex
GO:0006369 termination of RNA polymerase II transcription
GO:0006379 mRNA cleavage
GO:0031124 mRNA 3'-end processing
Regulatory
For research purposes only

Applications of PCF11 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg