PC4 antibody

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Rabbit anti PC4

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00736
Datasheet
WB
SDS
100 µl loader

Rabbit anti Human PC4 antibody recognizes the activated RNA polymerase II transcriptional coactivator p15, also known as activated RNA polymerase II transcription cofactor 4, or positive cofactor 4.

This protein forms a homodimer and is aneneral coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. It also may be involved in stabilizing the multiprotein transcription complex. SUB1 in vivo binds single stranded DNA, but in vitro, it binds non-specifically to double-stranded DNA (ds DNA).

Rabbit anti Human PC4 antibody detects a band of 17 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Rabbit anti PC4 detects a band of approximately 17 kDa in MCF7 cell lysates
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
2% Sucrose
Immunogen
Synthetic peptide encompassing part of the middle region of human PC4

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P53999
Entrez Gene
SUB1
GO Terms
GO:0005515 protein binding
GO:0003697 single-stranded DNA binding
GO:0003713 transcription coactivator activity
GO:0005667 transcription factor complex
GO:0005730 nucleolus
GO:0006357 regulation of transcription from RNA polymerase II promoter
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of PC4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml