Paxillin antibody
Rabbit anti Paxillin
Rabbit anti-human paxillin antibody recognizes paxillin. This antibody has been extensively validated for western blotting using whole cell lysates.
Paxillin Antibody with Control Lysate
Rabbit anti-human paxillin antibody recognizes paxillin. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00573 | WB | 100 µl | ||||
| VPA00573KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Rabbit anti Human paxillin antibody recognizes paxillin.
The PXN gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for PXN. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445) (provided by RefSeq, Aug 2011).
Rabbit anti Human paxillin antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The PXN gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for PXN. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445) (provided by RefSeq, Aug 2011).
Rabbit anti Human paxillin antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti paxillin detects a band of approximately 62 kDa in A431 cell lysates
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Rabbit polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Rabbit polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- KLH conjugated synthetic peptide corresponding to 94-125 amino acid from human paxillin
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg A431 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P49023 Related reagents
- Entrez Gene
- PXN Related reagents
- GO Terms
- GO:0006936 muscle contraction
- GO:0005737 cytoplasm
- GO:0005875 microtubule associated complex
- GO:0005925 focal adhesion
- GO:0007160 cell-matrix adhesion
- GO:0007172 signal complex assembly
- GO:0007173 epidermal growth factor receptor signaling pathway
- GO:0008013 beta-catenin binding
- GO:0008270 zinc ion binding
- GO:0017166 vinculin binding
- GO:0030027 lamellipodium
- GO:0034329 cell junction assembly
- GO:0034614 cellular response to reactive oxygen species
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Paxillin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR208P | 2 ml | WB |
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