Rabbit anti PAK2 (pSer20) antibody
recognizes serine/threonine-protein kinase PAK2, also known as p21-activated kinase2 or PAK65, when phosphorylated at serine 20. PAK2 belongs to the p21-activated protein kinase (PAK) family, which are targets of small GTPases CDC42 and RAC1, and regulate apoptosis and cell motility (Knaus, U.G. & Bokoch, G.M.1998
). In addition to autophosphorylation of PAK2 at serine 20 (Jung, J.H. & Traugh, J.A. 2005
), phosphorylation of this particular residue can also induced by PKG (Yurdagul A, J.r. et al. 2013
). Increased levels of PAK2 phosphorylated on serine 20 have been reported in ovarian cancer and implicated to play role in ovarian cancerogenesis (Siu, M.K. et al. 2010
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to PAK2 (pSer20) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding serine 20 of human PAK2
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
protein serine/threonine kinase activity
negative regulation of protein kinase activity
cellular component disassembly involved in apoptosis
identical protein binding
regulation of apoptosis
protein kinase binding
protein tyrosine kinase activator activity
T cell costimulation
regulation of growth
interspecies interaction between organisms
perinuclear region of cytoplasm
regulation of defense response to virus by virus
positive regulation of peptidyl-tyrosine phosphorylation
T cell receptor signaling pathway
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of PAK2 (pSer20) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Useful Reagents Available