PAK2 (pSer20) antibody

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Rabbit anti PAK2 (pSer20)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2703
Datasheet
WB
MSDS
50 µl loader

Rabbit anti PAK2 (pSer20) antibody recognizes serine/threonine-protein kinase PAK2, also known as p21-activated kinase2 or PAK65, when phosphorylated at serine 20. PAK2 belongs to the p21-activated protein kinase (PAK) family, which are targets of small GTPases CDC42 and RAC1, and regulate apoptosis and cell motility (Knaus, U.G. & Bokoch, G.M.1998). In addition to autophosphorylation of PAK2 at serine 20 (Jung, J.H. & Traugh, J.A. 2005), phosphorylation of this particular residue can also induced by PKG (Yurdagul A, J.r. et al. 2013). Increased levels of PAK2 phosphorylated on serine 20 have been reported in ovarian cancer and implicated to play role in ovarian cancerogenesis (Siu, M.K. et al. 2010).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to PAK2 (pSer20) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 20 of human PAK2

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q13177 Related reagents
Entrez Gene
PAK2 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006469 negative regulation of protein kinase activity
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0016032 viral reproduction
GO:0042981 regulation of apoptosis
GO:0018105 peptidyl-serine phosphorylation
GO:0019901 protein kinase binding
GO:0030296 protein tyrosine kinase activator activity
GO:0031295 T cell costimulation
GO:0040008 regulation of growth
GO:0044419 interspecies interaction between organisms
GO:0046777 protein autophosphorylation
GO:0048471 perinuclear region of cytoplasm
GO:0050690 regulation of defense response to virus by virus
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050852 T cell receptor signaling pathway
Regulatory
For research purposes only

Applications of PAK2 (pSer20) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti PAK2 (C-Terminal) AHP1191 P* WB 0.1 mg loader
Rabbit anti PAK2 AHP2505 P WB 50 µl loader
Rabbit anti PAK2 VPA00083 WB 100 µl loader