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PAK2 antibody

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Anti PAK2 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti PAK2 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2 Anti PAK2 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3
Anti PAK2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Rabbit anti Human PAK2 antibody (VPA00083) followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to PAK2 (molecular weight 58 kDa).
Anti PAK2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Formalin fixed, paraffin embedded mouse spleen stained with Rabbit anti Human PAK2 (VPA00083) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0).
Anti PAK2 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3
Western blot analysis of whole cell lysate from Jurkat human T-cell leukemia cells probed with Rabbit anti Human PAK2 (VPA00083) at 0.5(A) and 1(B) μg/ml.

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Rabbit anti PAK2

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
PAK2

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Product Code Applications Pack Size List Price Your Price Qty
VPA00083
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P * WB 100 µl loader
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Rabbit anti Human PAK2 antibody recognizes p21-activated kinase-2 (PAK2), a ubiquitously expressed, serine/threonine protease found at higher levels in skeletal muscle, ovary, thymus and spleen.

p21-activated kinases (PAKs) bind activated Cdc42 and Rac via a Cdc42/Rac interactive binding (CRIB) domain. Group 1 PAK kinases (PAK1, 2 and 3) regulate the activity of an adjacent conserved autoinhibitory domain and are associated with cellular processes, including cytoskeleton rearrangement, apoptosis and MAP kinase signalling. Group 2 PAKs (PAK4, 5, and 6) lack the autoinhibitory domain and are not activated by Cdc42/Rac binding.

PAK2 has a cytostatic effect on cells and is activated in response to cellular stresses such hyperosmolarity, serum starvation, contact inhibition and DNA damage from radiation and chemicals. Unlike other PAKs, PAK2 can be activated by proteolytic cleavage to generate a constitutively active fragment, PAK2p34, which induces a cell death response. This induction is thought to be regulated by Rho GTPase-activating protein 10 which inhibits the protein kinase activity of PAK2p34 and causes it to localise to the perinuclear region instead of the nucleus. PAK2 may also play a role in X-linked mental retardation.

Rabbit anti Human PAK2 antibody detects p21-activated kinase-2 as a single a band of 58 kDa in a range of human cell line lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Rabbit anti PAK2 detects a band of approximately 58 kDa in MCF7 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Rabbit polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
14 amino acid peptide sequence near the carboxy terminus of human PAK2.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1
Western Blotting 1/1000
  1. 1 This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rabbit IgG (H/L):HRP STAR208P WB 2 ml
List Price Your Price
Description Goat anti Rabbit IgG (H/L):HRP

Further Reading

  1. Roig, J. & Traugh, J.A. (2001) Cytostatic p21 G protein-activated protein kinase gamma-PAK.
    Vitam Horm. 62: 167-98.
  2. Rudel, T. & Bokoch, G.M. (1997) Membrane and morphological changes in apoptotic cells regulated by caspase-mediated activation of PAK2.
    Science. 276 (5318): 1571-4.
  3. Jaffer, Z.M. & Chernoff, J. (2002) p21-activated kinases: three more join the Pak.
    Int J Biochem Cell Biol. 34 (7): 713-7.
  4. Thome, M. et al. (1997) Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors.
    Nature. 386 (6624): 517-21.
  5. Irmler, M. et al. (1997) Inhibition of death receptor signals by cellular FLIP.
    Nature. 388 (6638): 190-5.
  6. Shu, H.B. et al. (1997) Casper is a FADD- and caspase-related inducer of apoptosis.
    Immunity. 6 (6): 751-63.
  7. Hu, S. et al. (1997) I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis.
    J Biol Chem. 272 (28): 17255-7.

Synonyms
p21 Activated Kinase 2
UniProt
Q13177
Entrez Gene
PAK2
GO Terms
GO:0005886 plasma membrane
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006469 negative regulation of protein kinase activity
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0016032 viral reproduction
GO:0042981 regulation of apoptosis
GO:0018105 peptidyl-serine phosphorylation
GO:0019901 protein kinase binding
GO:0030296 protein tyrosine kinase activator activity
GO:0031295 T cell costimulation
GO:0040008 regulation of growth
GO:0044419 interspecies interaction between organisms
GO:0046777 protein autophosphorylation
GO:0048471 perinuclear region of cytoplasm
GO:0050690 regulation of defense response to virus by virus
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050852 T cell receptor signaling pathway

VPA00083

131014

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