PAK1 (pThr84) antibody

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Rabbit anti PAK1 (pThr84)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2269 WB* 0.1 ml
Rabbit anti PAK1 (pThr84) antibody recognizes p21-activated kinases (PAK) 1, also known as serine/threonine-protein kinase PAK 1, alpha-PAK and p65-PAK, when phosphorylated at threonine 84.

Serine/threonine-protein kinase PAK1 is an enzyme that in humans is encoded by the PAK1 gene. PAK proteins are critical effectors that link the Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins are a family of serine/threonine p21-activated kinases and include PAK1-6. These proteins serve as targets for small GTP binding proteins such as OSR1.

OSR1, oxidative stress response 1, is activated by osmotic stresses including sorbitol or NaCl. It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at threonine 84 and inhibits activation of the JNK and MAPK signaling pathway.

PAK proteins also serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine Expected from Sequence
Dog Expected from Sequence
Chicken Expected from Sequence
Guinea Pig Expected from Sequence
Rat Expected from Sequence
Sheep Expected from Sequence
Zebrafish Expected from Sequence
Mouse
Primate Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to PAK1 (pThr84) was raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity purification via sequential chromatography on phospho- and dephospho-peptide columns
Buffer Solution
HEPES buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr84 (pThr84) region of human PAK1

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
Q13153 Related reagents
Entrez Gene
PAK1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0007010 cytoskeleton organization
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005518 collagen binding
GO:0005524 ATP binding
GO:0005794 Golgi apparatus
GO:0005925 focal adhesion
GO:0006984 ER-nucleus signaling pathway
GO:0007411 axon guidance
GO:0031295 T cell costimulation
GO:0043507 positive regulation of JUN kinase activity
GO:0046777 protein autophosphorylation
GO:0050852 T cell receptor signaling pathway
Regulatory
For research purposes only

Applications of PAK1 (pThr84) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1 1/1000
  1. 1 For the detection of phosphoproteins, phosphatase inhibitors should always be added freshly to the lysis buffer. Milk or other casein-based blocking solutions are generally not recommended for blocking; as casein is itself a phosphoprotein its use can result in high background.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti Human PAK1 (pThr84) detects a band at approximately 68 kDa in mouse hippocampal lysate

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB