PAK1 (pThr84) antibody
Rabbit anti PAK1 (pThr84) antibody recognizes p21-activated kinases (PAK) 1, also known as serine/threonine-protein kinase PAK 1, alpha-PAK and p65-PAK, when phosphorylated at threonine 84.
Serine/threonine-protein kinase PAK1 is an enzyme that in humans is encoded by the PAK1 gene. PAK proteins are critical effectors that link the Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins are a family of serine/threonine p21-activated kinases and include PAK1-6. These proteins serve as targets for small GTP binding proteins such as OSR1.
OSR1, oxidative stress response 1, is activated by osmotic stresses including sorbitol or NaCl. It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at threonine 84 and inhibits activation of the JNK and MAPK signaling pathway.
PAK proteins also serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology.
Serine/threonine-protein kinase PAK1 is an enzyme that in humans is encoded by the PAK1 gene. PAK proteins are critical effectors that link the Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins are a family of serine/threonine p21-activated kinases and include PAK1-6. These proteins serve as targets for small GTP binding proteins such as OSR1.
OSR1, oxidative stress response 1, is activated by osmotic stresses including sorbitol or NaCl. It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at threonine 84 and inhibits activation of the JNK and MAPK signaling pathway.
PAK proteins also serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Expected from Sequence Dog Expected from Sequence Chicken Expected from Sequence Guinea Pig Expected from Sequence Rat Expected from Sequence Sheep Expected from Sequence Zebrafish Expected from Sequence Mouse Primate Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to PAK1 (pThr84) was raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity purification via sequential chromatography on phospho- and dephospho-peptide columns
- Buffer Solution
- HEPES buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr84 (pThr84) region of human PAK1
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- Q13153 Related reagents
- Entrez Gene
- PAK1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0007010 cytoskeleton organization
- GO:0006915 apoptosis
- GO:0005829 cytosol
- GO:0004674 protein serine/threonine kinase activity
- GO:0005518 collagen binding
- GO:0005524 ATP binding
- GO:0005794 Golgi apparatus
- GO:0005925 focal adhesion
- GO:0006984 ER-nucleus signaling pathway
- GO:0007411 axon guidance
- GO:0031295 T cell costimulation
- GO:0043507 positive regulation of JUN kinase activity
- GO:0046777 protein autophosphorylation
- GO:0050852 T cell receptor signaling pathway
- Regulatory
- For research purposes only
Applications of PAK1 (pThr84) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting 1 | 1/1000 |
- 1 For the detection of phosphoproteins, phosphatase inhibitors should always be added freshly to the lysis buffer. Milk or other casein-based blocking solutions are generally not recommended for blocking; as casein is itself a phosphoprotein its use can result in high background.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti Human PAK1 (pThr84) detects a band at approximately 68 kDa in mouse hippocampal lysate
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
