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PAI-1 antibody

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Anti PAI-1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti PAI-1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2 Anti PAI-1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3 Anti PAI-1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 4 Anti PAI-1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 5
Anti PAI-1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Goat anti Human (VPA00047) PAI-1 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/50,000, STAR88P) and visualized on the ChemiDoc MP with 2 second exposure. Arrow points to PAI-1 (molecular weight 43 kDa).
Anti PAI-1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Western blot analysis of human liver lysate probed with Goat anti Human PAI-1 antibody (VPA00047).
Anti PAI-1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3
Immunohistochemical staining of formalin fixed, paraffin embedded human placenta stained with Goat anti Human PAI-1 antibody (VPA00047) following heat mediated antigen retrieval in citrate buffer (pH6.0).
Anti PAI-1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 4
Immunohistochemical staining of formalin fixed, paraffin embedded human placenta stained with Goat anti Human PAI-1 antibody (VPA00047) following heat mediated antigen retrieval in Tris/EDTA buffer (pH9.0).
Anti PAI-1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 5
ELISA analysis of human PAI-1 expression using Goat anti Human PAI-1 antibody (VPA00047) at1.5μml-1 as a detection reagent in a sandwich ELISA configuration.

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Goat anti PAI-1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
PAI-1

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Product Code Applications Pack Size List Price Your Price Qty
VPA00047
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P WB 100 µl
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Goat anti Human PAI-1 antibody recognizes human plasminogen activator inhibitor 1 (PAI-1), also known as Serpin E1. PAI-1 is a 402 amino acid serine protease inhibitor and the principle inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA)

PAI-1 is present in plasma, platelets and endothelial cells, is expressed at increased levels in various disease states (Kruithof et al. 1988), and has been linked to an increased occurrence of thrombosis in obesity and coronary artery disease (Oseroff et al. 1989). Defects in PAI-1 are characterized by abnormal bleeding (Takahashi et al. 1996).

Goat anti Human PAI-1 antibody recognizes PAI-1 as a single band of 43 kDa in a range of human cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Goat anti PAI-1 detects a band of approximately 43 kDa in MCF7 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide with sequence C-GFKIDDKGMAPALRH, corresponding to the internal region of PAI-1.
Approx. Protein Concentrations
0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

References for PAI-1 antibody

  1. De, B.T. et al. (2005) Plasminogen activator inhibitor-1: a common denominator in obesity, diabetes and cardiovascular disease.
    Curr Opin Pharmacol. 5 (2): 149-54.
  2. Berg, D. et al. (2012) Profiling signalling pathways in formalin-fixed and paraffin-embedded breast cancer tissues reveals cross-talk between EGFR, HER2, HER3 and uPAR.
    J Cell Physiol. 227 (1): 204-12.
  3. Wolff, C. et al. (2011) Signalling networks associated with urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 in breast cancer tissues: new insights from protein microarray analysis.
    J Pathol. 223 (1): 54-63.
  4. Malinowsky, K. et al. (2012) uPA and PAI-1-Related Signaling Pathways Differ between Primary Breast Cancers and Lymph Node Metastases.
    Transl Oncol. 5 (2): 98-104.
  5. Berg, D. et al. (2011) Discovery of new molecular subtypes in oesophageal adenocarcinoma.
    PLoS One. 6 (9): e23985.

Further Reading

  1. Lijnen, H.R. (2005) Pleiotropic functions of plasminogen activator inhibitor-1.
    J Thromb Haemost. 3 (1): 35-45.

UniProt
P05121
Entrez Gene
SERPINE1
GO Terms
GO:0005886 plasma membrane
GO:0001300 chronological cell aging
GO:0002020 protease binding
GO:0002576 platelet degranulation
GO:0004252 serine-type endopeptidase activity
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0005615 extracellular space
GO:0050829 defense response to Gram-negative bacterium
GO:0010469 regulation of receptor activity
GO:0010757 negative regulation of plasminogen activation
GO:0014912 negative regulation of smooth muscle cell migration
GO:0030168 platelet activation
GO:0031012 extracellular matrix
GO:0031093 platelet alpha granule lumen
GO:0032757 positive regulation of interleukin-8 production
GO:0033629 negative regulation of cell adhesion mediated by integrin
GO:0035491 positive regulation of leukotriene production involved in inflammatory response
GO:0042730 fibrinolysis
GO:0043066 negative regulation of apoptosis
GO:0045766 positive regulation of angiogenesis
GO:0048260 positive regulation of receptor-mediated endocytosis
GO:0051918 negative regulation of fibrinolysis
GO:0061044 negative regulation of vascular wound healing
GO:0070887 cellular response to chemical stimulus
GO:0071222 cellular response to lipopolysaccharide
GO:0090026 positive regulation of monocyte chemotaxis
GO:2000098 negative regulation of smooth muscle cell-matrix adhesion

VPA00047

230914

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