PAI-1 antibody

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Goat anti Human PAI-1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1100 E P* WB 0.1 mg
Goat anti human PAI-1 antibody recognizes human Plasminogen activator inhibitor 1 (PAI-1), also known as Serpin E1. PAI-1 is a 402 amino acid }45kDa serine protease inhibitor epitope within the internal region of human Serpin E1. PAI-1 (Serpin E1) is a 45kD serine protease inhibitor and the principle inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA)

PAI-1 is present in plasma, platelets and endothelial cells, is expressed at increased levels in various disease states (Kruithof et al. 1988), and has been linked to an increased occurrence of thrombosis in obesity and coronary artery disease (Oseroff et al. 1989). Defects in PAI-1 are characterized by abnormal bleeding (Takahashi et al. 1996).

Goat anti human PAI-1 antibody has been used successfully for the detection of PAI-1 in human tissues by immunohistochemistry, western blotting and ELISA.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Dog Expected from Sequence
Chimpanzee Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human PAI-1 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Peptide with sequence C-GFKIDDKGMAPALRH, corresponding to the internal region of PAI-1 (NP_000593.1).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P05121 Related reagents
Entrez Gene
SERPINE1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001300 chronological cell aging
GO:0002020 protease binding
GO:0002576 platelet degranulation
GO:0004252 serine-type endopeptidase activity
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0005615 extracellular space
GO:0050829 defense response to Gram-negative bacterium
GO:0010469 regulation of receptor activity
GO:0010757 negative regulation of plasminogen activation
GO:0014912 negative regulation of smooth muscle cell migration
GO:0030168 platelet activation
GO:0031012 extracellular matrix
GO:0031093 platelet alpha granule lumen
GO:0032757 positive regulation of interleukin-8 production
GO:0033629 negative regulation of cell adhesion mediated by integrin
GO:0035491 positive regulation of leukotriene production involved in inflammatory response
GO:0042730 fibrinolysis
GO:0043066 negative regulation of apoptosis
GO:0045766 positive regulation of angiogenesis
GO:0048260 positive regulation of receptor-mediated endocytosis
GO:0051918 negative regulation of fibrinolysis
GO:0061044 negative regulation of vascular wound healing
GO:0070887 cellular response to chemical stimulus
GO:0071222 cellular response to lipopolysaccharide
GO:0090026 positive regulation of monocyte chemotaxis
GO:2000098 negative regulation of smooth muscle cell-matrix adhesion
Regulatory
For research purposes only

Applications of PAI-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/32000
Immunohistology - Paraffin 1 4.0 6.0 ug/ml
Western Blotting 0.1 0.3 ug/ml
  1. 1 This antibody requires heat-mediated antigen retrieval prior to staining paraffin sections. Citrate buffer pH6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human Placenta
Western Blotting
Goat anti human PAI-1 detects a band of approximately 45kDa in human duodenum, liver and lung lysates. A minimum incubation time of 1 hour is recommended with this antibody.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Product Specific References

References for PAI-1 antibody

  1. De Taeye, B. et al. (2005) Plasminogen activator inhibitor-1: a common denominator in obesity, diabetes and cardiovascular disease.
    Curr Opin Pharmacol. 5 (2): 149-54.
  2. Berg, D. et al. (2012) Profiling signalling pathways in formalin-fixed and paraffin-embedded breast cancer tissues reveals cross-talk between EGFR, HER2, HER3 and uPAR.
    J Cell Physiol. 227: 204-12.
  3. Wolff, C. et al. (2011) Signalling networks associated with urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 in breast cancer tissues: new insights from protein microarray analysis.
    J Pathol. 223: 54-63.
  4. Malinowsky, K. et al. (2014) uPA and PAI-1-Related Signaling Pathways Differ between Primary Breast Cancers and Lymph Node Metastases.
    Transl Oncol. 5: 98-104.
  5. Berg, D. et al. (2011) Discovery of new molecular subtypes in oesophageal adenocarcinoma.
    PLoS One. 6 (9): e23985.

Further Reading

  1. Lijnen, H.R. (2005) Pleiotropic functions of plasminogen activator inhibitor-1.
    J Thromb Haemost. 3 (1): 35-45.