PAI-1 antibody
Goat anti human PAI-1 antibody recognizes an internal epitope within human plasminogen activator inhibitor 1 (PAI-1), also known as serpin E1. PAI-1 is a 402 amino acid ~45 kDa serine protease inhibitor and the principle inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA)
PAI-1 is present in plasma, platelets and endothelial cells, is expressed at increased levels in various disease states (Kruithof et al. 1988), and has been linked to an increased occurrence of thrombosis in obesity and coronary artery disease (Oseroff et al. 1989). Defects in PAI-1 are characterized by abnormal bleeding (Takahashi et al. 1996).
Goat anti human PAI-1 antibody has been used successfully for the detection of PAI-1 in human tissues by immunohistochemistry, western blotting and ELISA.
PAI-1 is present in plasma, platelets and endothelial cells, is expressed at increased levels in various disease states (Kruithof et al. 1988), and has been linked to an increased occurrence of thrombosis in obesity and coronary artery disease (Oseroff et al. 1989). Defects in PAI-1 are characterized by abnormal bleeding (Takahashi et al. 1996).
Goat anti human PAI-1 antibody has been used successfully for the detection of PAI-1 in human tissues by immunohistochemistry, western blotting and ELISA.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Dog Expected from Sequence Chimpanzee Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human PAI-1 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
0.02% Sodium Azide 0.5% Bovine Serum Albumin - Immunogen
- Peptide with sequence C-GFKIDDKGMAPALRH, corresponding to the internal region of PAI-1 (NP_000593.1).
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P05121
- Entrez Gene
- SERPINE1
- GO Terms
- GO:0005886 plasma membrane
- GO:0001300 chronological cell aging
- GO:0002020 protease binding
- GO:0002576 platelet degranulation
- GO:0004252 serine-type endopeptidase activity
- GO:0004867 serine-type endopeptidase inhibitor activity
- GO:0005615 extracellular space
- GO:0050829 defense response to Gram-negative bacterium
- GO:0010469 regulation of receptor activity
- GO:0010757 negative regulation of plasminogen activation
- GO:0014912 negative regulation of smooth muscle cell migration
- GO:0030168 platelet activation
- GO:0031012 extracellular matrix
- GO:0031093 platelet alpha granule lumen
- GO:0032757 positive regulation of interleukin-8 production
- GO:0033629 negative regulation of cell adhesion mediated by integrin
- GO:0035491 positive regulation of leukotriene production involved in inflammatory response
- GO:0042730 fibrinolysis
- GO:0043066 negative regulation of apoptosis
- GO:0045766 positive regulation of angiogenesis
- GO:0048260 positive regulation of receptor-mediated endocytosis
- GO:0051918 negative regulation of fibrinolysis
- GO:0061044 negative regulation of vascular wound healing
- GO:0070887 cellular response to chemical stimulus
- GO:0071222 cellular response to lipopolysaccharide
- GO:0090026 positive regulation of monocyte chemotaxis
- GO:2000098 negative regulation of smooth muscle cell-matrix adhesion
- Regulatory
- For research purposes only
Applications of PAI-1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/32000 | ||
| Immunohistology - Paraffin 1 | 4.0 | 6.0 ug/ml | |
| Western Blotting | 0.1 | 0.3 ug/ml |
- 1 This antibody requires heat-mediated antigen retrieval prior to staining paraffin sections. Citrate buffer pH6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human Placenta
- Western Blotting
- Goat anti human PAI-1 detects a band of approximately 45kDa in human duodenum, liver and lung lysates. A minimum incubation time of 1 hour is recommended with this antibody.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | F | 1 mg |
|
|
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | C E WB | 1 mg |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
Product Specific References
References for PAI-1 antibody
-
De Taeye, B. et al. (2005) Plasminogen activator inhibitor-1: a common denominator in obesity, diabetes and cardiovascular disease.
Curr Opin Pharmacol. 5 (2): 149-54. -
Berg, D. et al. (2012) Profiling signalling pathways in formalin-fixed and paraffin-embedded breast cancer tissues reveals cross-talk between EGFR, HER2, HER3 and uPAR.
J Cell Physiol. 227: 204-12. -
Wolff, C. et al. (2011) Signalling networks associated with urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 in breast cancer tissues: new insights from protein microarray analysis.
J Pathol. 223: 54-63. -
Malinowsky, K. et al. (2014) uPA and PAI-1-Related Signaling Pathways Differ between Primary Breast Cancers and Lymph Node Metastases.
Transl Oncol. 5: 98-104. -
Berg, D. et al. (2011) Discovery of new molecular subtypes in oesophageal adenocarcinoma.
PLoS One. 6 (9): e23985.
Further Reading
-
Lijnen, H.R. (2005) Pleiotropic functions of plasminogen activator inhibitor-1.
J Thromb Haemost. 3 (1): 35-45.
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