PAI-1 antibody

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Anti Human PAI-1 Antibody thumbnail image 2

Anti Human PAI-1 Antibody thumbnail image 3

Anti Human PAI-1 Antibody thumbnail image 4

Anti Human PAI-1 Antibody gallery image 1 — Western blot analysis of human liver lysate probed with Goat anti Human PAI-1 antibody (**AHP1100**).

Anti Human PAI-1 Antibody gallery image 2 — Immunohistochemical staining of formalin fixed, paraffin embedded human placenta stained with Goat anti Human PAI-1 antibody (**AHP1100**) following heat mediated antigen retrieval in citrate buffer (pH6.0).

Anti Human PAI-1 Antibody gallery image 3 — Immunohistochemical staining of formalin fixed, paraffin embedded human placenta stained with Goat anti Human PAI-1 antibody (**AHP1100**) following heat mediated antigen retrieval in Tris/EDTA buffer (pH9.0).

Anti Human PAI-1 Antibody gallery image 4 — ELISA analysis of human PAI-1 expression using Goat anti Human PAI-1 antibody (**AHP1100**) at1.5μml^-1 as a detection reagent in a sandwich ELISA configuration.

Goat anti Human PAI-1

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP1100 Datasheet	E P* WB MSDS	0.1 mg	Log in		Get Quote

Goat anti human PAI-1 antibody recognizes human Plasminogen activator inhibitor 1 (PAI-1), also known as Serpin E1. PAI-1 is a 402 amino acid }45kDa serine protease inhibitor epitope within the internal region of human Serpin E1. PAI-1 (Serpin E1) is a 45kD serine protease inhibitor and the principle inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA)

PAI-1 is present in plasma, platelets and endothelial cells, is expressed at increased levels in various disease states (Kruithof et al. 1988), and has been linked to an increased occurrence of thrombosis in obesity and coronary artery disease (Oseroff et al. 1989). Defects in PAI-1 are characterized by abnormal bleeding (Takahashi et al. 1996).

Goat anti human PAI-1 antibody has been used successfully for the detection of PAI-1 in human tissues by immunohistochemistry, western blotting and ELISA.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Dog	Expected from Sequence
Chimpanzee	Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human PAI-1 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02%	Sodium Azide
0.5%	Bovine Serum Albumin

Immunogen

Peptide with sequence C-GFKIDDKGMAPALRH, corresponding to the internal region of PAI-1 (NP_000593.1).

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: P05121 Related reagents
Entrez Gene: SERPINE1 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0001300 chronological cell aging; GO:0002020 protease binding; GO:0002576 platelet degranulation; GO:0004252 serine-type endopeptidase activity; GO:0004867 serine-type endopeptidase inhibitor activity; GO:0005615 extracellular space; GO:0050829 defense response to Gram-negative bacterium; GO:0010469 regulation of receptor activity; GO:0010757 negative regulation of plasminogen activation; GO:0014912 negative regulation of smooth muscle cell migration; GO:0030168 platelet activation; GO:0031012 extracellular matrix; GO:0031093 platelet alpha granule lumen; GO:0032757 positive regulation of interleukin-8 production; GO:0033629 negative regulation of cell adhesion mediated by integrin; GO:0035491 positive regulation of leukotriene production involved in inflammatory response; GO:0042730 fibrinolysis; GO:0043066 negative regulation of apoptosis; GO:0045766 positive regulation of angiogenesis; GO:0048260 positive regulation of receptor-mediated endocytosis; GO:0051918 negative regulation of fibrinolysis; GO:0061044 negative regulation of vascular wound healing; GO:0070887 cellular response to chemical stimulus; GO:0071222 cellular response to lipopolysaccharide; GO:0090026 positive regulation of monocyte chemotaxis; GO:2000098 negative regulation of smooth muscle cell-matrix adhesion
Regulatory: For research purposes only

Applications of PAI-1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA		1/32000
Immunohistology - Paraffin ¹	4.0	6.0 ug/ml
Western Blotting	0.1	0.3 ug/ml

¹ This antibody requires heat-mediated antigen retrieval prior to staining paraffin sections. Citrate buffer pH6.0 is recommended for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Human Placenta
Western Blotting: Goat anti human PAI-1 detects a band of approximately 45kDa in human duodenum, liver and lung lysates. A minimum incubation time of 1 hour is recommended with this antibody.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	F	1 mg	Log in		Get Quote
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C E WB	1 mg	Log in		Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in		Get Quote
Antigen Retrieval Buffer, pH8.0	BUF025C	P	50 ml	Log in		Get Quote

Product Specific References

References for PAI-1 antibody

De Taeye, B. et al. (2005) Plasminogen activator inhibitor-1: a common denominator in obesity, diabetes and cardiovascular disease.
Curr Opin Pharmacol. 5 (2): 149-54.
Berg, D. et al. (2012) Profiling signalling pathways in formalin-fixed and paraffin-embedded breast cancer tissues reveals cross-talk between EGFR, HER2, HER3 and uPAR.
J Cell Physiol. 227: 204-12.
Wolff, C. et al. (2011) Signalling networks associated with urokinase-type plasminogen activator (uPA) and its inhibitor PAI-1 in breast cancer tissues: new insights from protein microarray analysis.
J Pathol. 223: 54-63.
Malinowsky, K. et al. (2014) uPA and PAI-1-Related Signaling Pathways Differ between Primary Breast Cancers and Lymph Node Metastases.
Transl Oncol. 5: 98-104.
Berg, D. et al. (2011) Discovery of new molecular subtypes in oesophageal adenocarcinoma.
PLoS One. 6 (9): e23985.