PACE4 antibody

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Rabbit anti Human PACE4

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1110 P 50 µg
Rabbit anti Human PACE4 antibody detects an epitope within the internal region of human Paired basic amino acid cleaving system 4 (PACE4). PACE4 belongs to the subtilisin-like proprotein convertase family, and is a ~100 kDa calcium dependent serine endoprotease located in the endoplasmic reticulum. It cleaves its substrates at their paired basic amino acid processing sites, converting them into their biologically active products. Several PACE4 substrates have been identified, including transforming growth factor beta-related proteins, proalbumin and von Willebrand factor.

In mouse, PACE4 has been shown to increase tumor cell invasiveness, and thus enhance tumor progression. It is thought to have a similar role in humans (Mahloogi et al. 2002).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to PACE4 were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to an internal domain of human PACE4.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P29122 Related reagents
Entrez Gene
PCSK6 Related reagents
Regulatory
For research purposes only

Applications of PACE4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 15 25ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Brain, cortex.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P

Product Specific References

Further Reading

  1. Gensberg, K. et al. (1998) Subtilisin-related serine proteases in the mammalian constitutive secretory pathway.
    Semin Cell Dev Biol. 9 (1): 11-7.
  2. Khatib, A.M. et al. (2002) Proprotein convertases in tumor progression and malignancy: novel targets in cancer therapy.
    Am J Pathol. 160 (6): 1921-35.