P90RSK1 antibody

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Rabbit anti P90RSK1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2710
Datasheet
WB
SDS
50 µl loader

Rabbit anti P90RSK1 antibody recognizes recognizes ribosomal protein S6 kinase alpha-1, also known as p90S6K, MAPK-activated protein kinase 1a, MAPKAPK-1a and RSK-1. It belongs to the RSK (ribosomal S6 kinase) family of serine/threonine kinases (Fisher and Blenis 1996). It is a downstream effector of Ras/ERK signalling pathway (Casalvieri et al. 2017). It directly phosphorylates IkappaBbeta and activates NF-kappaB (Xu et al. 2006). It has been reported to phosphorylate CREB on Ser133 (Cho et al. 2011). RSK also promotes mTOR signaling and translation (Shahbazian et al. 2006). Expression and activity of RSK1 promotes tumor growth and survival (Casalvieri et al. 2017).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to P90RSK1 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
A synthetic peptide corresponding to a sequence from amino acid 600 to the C-terminus of human P90RSK1

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q15418
Entrez Gene
RPS6KA1
GO Terms
GO:0005515 protein binding
GO:0000287 magnesium ion binding
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0005829 cytosol
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006468 protein phosphorylation
GO:0007268 synaptic transmission
GO:0007411 axon guidance
GO:0008063 Toll signaling pathway
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0051403 stress-activated MAPK cascade
Regulatory
For research purposes only

Applications of P90RSK1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti P90RSK1 antibody detects a band of approximately 90 kDa in various cell and tissue lysates under reducing conditions

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg