p70S6K antibody

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Anti Human p70S6K (C-Terminal) Antibody thumbnail image 1

Anti Human p70S6K (C-Terminal) Antibody thumbnail image 2

Anti Human p70S6K (C-Terminal) Antibody thumbnail image 3

Anti Human p70S6K (C-Terminal) Antibody thumbnail image 4

Anti Human p70S6K (C-Terminal) Antibody gallery image 1 — Immunohistochemical staining of paraffin embedded human kidney following heat mediated antigen retrieval with citrate buffer at pH 6.0 using Goat anti p70S6K (**AHP1575**) at 2.0μgml^-1, followed by anti Goat - HRP staining and DAB chromogen development

Anti Human p70S6K (C-Terminal) Antibody gallery image 2 — Western blotting analysis of human placenta lysate (RIPA buffer, 35μg total protein per lane) probed with Goat anti Human p70S6K antibody (**AHP1575**) used at 0.05μgml^-1. Primary incubated for 1 hour. Signal was detected by western blot using chemiluminescence

Anti Human p70S6K (C-Terminal) Antibody gallery image 3 — Western blotting analysis of rat brain lysate (35μg protein in RIPA buffer) probed with Goat anti Human p70S6K antibody (**AHP1575**) used at 1.0μgml^-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Anti Human p70S6K (C-Terminal) Antibody gallery image 4 — Western blotting analysis of NIH3T3 cell lysate (35μg protein in RIPA buffer) probed with Goat anti p70S6K antibody (**AHP1575**) used at 0.1μgml^-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.

Goat anti Human p70S6K (C-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AHP1575 Datasheet	E WB SDS	0.1 mg	Log in		Get Quote

Goat anti phospho-70S6K antibody recognizes an epitope within the C-terminal (CT) region of human p70S6K, otherwise known as RPS6KB1 or Ribosomal protein S6 kinase beta-1. 70S6K is a serine/threonine kinase which acts upon 40S ribosomal protein S6 to induce protein synthesis, allowing for efficient G1 phase progression.

p70S6K is activated following phosphorylation of several sites, in response to hormones, growth factors, mitogenic stimuli and physical exercise, and is a downstream effector in the AKT/mTOR (mammalian target of rapamycin) signalling pathway. p70S6K also acts as a regulator of cell motility, invasion, and cytoskeletal reorganization.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat	Expected from Sequence
Mouse	Expected from Sequence
Rabbit	Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to human p70S6K (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin

Immunogen

Peptide sequence C-MISKRPEHLRMNL corresponding to the C-Terminal region of p70S6K (NP_003152.1).

Approx. Protein Concentrations

IgG concentration 0.5mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P23443
Entrez Gene: RPS6KB1
GO Terms: GO:0000082 G1/S transition of mitotic cell cycle; GO:0005515 protein binding; GO:0006915 apoptosis; GO:0005829 cytosol; GO:0004672 protein kinase activity; GO:0005524 ATP binding; GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0005741 mitochondrial outer membrane; GO:0008286 insulin receptor signaling pathway; GO:0019717 synaptosome; GO:0048015 phosphatidylinositol-mediated signaling; GO:0030054 cell junction; GO:0031929 TOR signaling cascade; GO:0043066 negative regulation of apoptosis; GO:0045202 synapse; GO:0045931 positive regulation of mitotic cell cycle; GO:0045948 positive regulation of translational initiation
Regulatory: For research purposes only

Applications of p70S6K antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			1/128000
Western Blotting		0.2	1.0ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP1575 detects a band of approximately 65-70kDa in human muscle and placenta cell lysates.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Rabbit anti Goat IgG (Fc):FITC	STAR122F	F	1 mg	Log in		Get Quote
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C E WB	1 mg	Log in		Get Quote

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