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p70S6K antibody

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Anti Human p70S6K (C-Terminal) Antibody thumbnail image 1 Anti Human p70S6K (C-Terminal) Antibody thumbnail image 2 Anti Human p70S6K (C-Terminal) Antibody thumbnail image 3 Anti Human p70S6K (C-Terminal) Antibody thumbnail image 4
Anti Human p70S6K (C-Terminal) Antibody gallery image 1
Western blotting analysis of human placenta lysate (RIPA buffer, 35μg total protein per lane) probed with Goat anti Human p70S6K antibody (AHP1575) used at 0.05μgml-1. Primary incubated for 1 hour. Signal was detected by western blot using chemiluminescence
Anti Human p70S6K (C-Terminal) Antibody gallery image 2
Western blotting analysis of rat brain lysate (35μg protein in RIPA buffer) probed with Goat anti Human p70S6K antibody (AHP1575) used at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.
Anti Human p70S6K (C-Terminal) Antibody gallery image 3
Western blotting analysis of NIH3T3 cell lysate (35μg protein in RIPA buffer) probed with Goat anti p70S6K antibody (AHP1575) used at 0.1μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence.
Anti Human p70S6K (C-Terminal) Antibody gallery image 4
Immunohistochemical staining of paraffin embedded human kidney following heat mediated antigen retrieval with citrate buffer at pH 6.0 using Goat anti p70S6K (AHP1575) at 2.0μgml-1, followed by anti Goat - HRP staining and DAB chromogen development

Goat anti Human p70S6K (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1575
Datasheet
E WB
SDS
0.1 mg

Goat anti phospho-70S6K antibody recognizes an epitope within the C-terminal (CT) region of human p70S6K, otherwise known as RPS6KB1 or Ribosomal protein S6 kinase beta-1. 70S6K is a serine/threonine kinase which acts upon 40S ribosomal protein S6 to induce protein synthesis, allowing for efficient G1 phase progression.

p70S6K is activated following phosphorylation of several sites, in response to hormones, growth factors, mitogenic stimuli and physical exercise, and is a downstream effector in the AKT/mTOR (mammalian target of rapamycin) signalling pathway. p70S6K also acts as a regulator of cell motility, invasion, and cytoskeletal reorganization.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
Mouse Expected from Sequence
Rabbit Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human p70S6K (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-MISKRPEHLRMNL corresponding to the C-Terminal region of p70S6K (NP_003152.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P23443
Entrez Gene
RPS6KB1
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0004672 protein kinase activity
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005741 mitochondrial outer membrane
GO:0008286 insulin receptor signaling pathway
GO:0019717 synaptosome
GO:0048015 phosphatidylinositol-mediated signaling
GO:0030054 cell junction
GO:0031929 TOR signaling cascade
GO:0043066 negative regulation of apoptosis
GO:0045202 synapse
GO:0045931 positive regulation of mitotic cell cycle
GO:0045948 positive regulation of translational initiation
Regulatory
For research purposes only

Applications of p70S6K antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/128000
Western Blotting 0.2 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1575 detects a band of approximately 65-70kDa in human muscle and placenta cell lysates.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

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