Rabbit anti p53 (pThr18) antibody
recognizes the p53 tumor suppressor protein, also known as cellular tumor antigen p53 or NY-CO-13, when phosphorylated at threonine 18. The p53 protein is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells and thus functions as a tumor suppressor, involved in preventing cancer (Haupt et al. 2003
). Mutations in the p53 gene are found in approximately half the cases of human cancer (Joerger and Fersht 2007
Phosphorylation of threonine 18 interferes with the binding of p53 to the E3 ubiquitin protein ligase MDM2 (Blanco et al. 2006
) promoting the apoptotic effects of p53.
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to p53 (pThr18) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
- Phospho specific-peptide corresponding to residues surrounding threonine 18 of human p53
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of p53 (pThr18) antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
Product Specific References
Haupt, S. et al. (2003) Apoptosis - the p53 network.
J Cell Sci. 116 (Pt 20): 4077-85.
Joerger, A.C. & Fersht, A.R. (2007) Structure-function-rescue: the diverse nature of common p53 cancer mutants.
Oncogene. 26 (15): 2226-42.
Blanco, S. et al. (2006) The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines.
FEBS J. 273 (11): 2487-504.
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