p53 (pSer9) antibody
UV and Serum Treatment: Cells were treated with UV for 15-30 minutes then cultured with serum for 1 hour
UV Treatment: Cells were treated with UV for 15-30 minutes.
Filter by Application:
WB ResetRabbit anti p53 (pSer9)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- p53 (pSer9)
Antibody Quality and Performance Guarantee
| Rabbit anti p53 (pSer9) antibody recognizes the p53 tumor suppressor protein, also known as cellular tumor antigen p53 or NY-CO-13, when phosphorylated at serine 9. The p53 protein is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells and thus functions as a tumor suppressor, involved in preventing cancer (Haupt et al. 2003). Mutations in the p53 gene are found in approximately half the cases of human cancer (Joerger and Fersht 2007). p53 is phosphorylated at serine 9 by CK1-δ (Higashimoto et al. 2000). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to p53 (pSer9) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 9 of human p53
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Further Reading
-
Haupt, S. et al. (2003) Apoptosis - the p53 network.
J Cell Sci. 116 (Pt 20): 4077-85. -
Joerger, A.C. & Fersht, A.R. (2007) Structure-function-rescue: the diverse nature of common p53 cancer mutants.
Oncogene. 26 (15): 2226-42. -
Higashimoto Y et al. (2000) Human p53 is phosphorylated on serines 6 and 9 in response to DNA damage-inducing agents.
J Biol Chem. 275 (30): 23199-203.
AHP2667
141624
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