p53 (pSer6) antibody
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Rabbit anti p53 (pSer6) antibody recognizes the p53 tumor suppressor protein, also known as cellular tumor antigen p53 or NY-CO-13, when phosphorylated at serine 6. The p53 protein is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells and thus functions as a tumor suppressor, involved in preventing cancer (Haupt et al. 2003). Mutations in the p53 gene are found in approximately half the cases of human cancer (Joerger and Fersht 2007).
p53 is phosphorylated at serine 6 in response to DNA damage (Higashimoto et al. 2000).
p53 is phosphorylated at serine 6 in response to DNA damage (Higashimoto et al. 2000).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to p53 (pSer6) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding serine 6 of human p53
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P04637
- Entrez Gene
- TP53
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of p53 (pSer6) antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Haupt, S. et al. (2003) Apoptosis - the p53 network.
J Cell Sci. 116 (Pt 20): 4077-85. -
Joerger, A.C. & Fersht, A.R. (2007) Structure-function-rescue: the diverse nature of common p53 cancer mutants.
Oncogene. 26 (15): 2226-42. -
Higashimoto, Y. et al. (2000) Human p53 is phosphorylated on serines 6 and 9 in response to DNA damage-inducing agents.
J Biol Chem. 275 (30): 23199-203.
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