p53 (pSer46) antibody

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Rabbit anti p53 (pSer46)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2665
Datasheet
WB
MSDS
50 µl loader

Rabbit anti p53 (pSer46) antibody recognizes the p53 tumor suppressor protein, also known as cellular tumor antigen p53 or NY-CO-13, when phosphorylated at serine 46. The p53 protein is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells and thus functions as a tumor suppressor, involved in preventing cancer (Haupt et al. 2003). Mutations in the p53 gene are found in approximately half the cases of human cancer (Joerger and Fersht 2007).

p53 is phosphorylated at serine 46 in response to DNA damage. Phosphorylation of p53 at serine 46 is important for the regulation of p53 mediated apoptosis (Taira et al. 2007).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to p53 (pSer46) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 46 of human p53

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P04637 Related reagents
Entrez Gene
TP53 Related reagents
Regulatory
For research purposes only

Applications of p53 (pSer46) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B C E P WB 1 ml loader
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg loader
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Haupt, S. et al. (2003) Apoptosis - the p53 network.
    J Cell Sci. 116 (Pt 20): 4077-85.
  2. Joerger, A.C. & Fersht, A.R. (2007) Structure-function-rescue: the diverse nature of common p53 cancer mutants.
    Oncogene. 26 (15): 2226-42.
  3. Taira, N. et al. (2007) DYRK2 is targeted to the nucleus and controls p53 via Ser46 phosphorylation in the apoptotic response to DNA damage.
    Mol Cell. 25 (5): 725-38.